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A3176

Sigma-Aldrich

α-淀粉酶 来源于猪胰腺

greener alternative

Type VI-B, ≥5 units/mg solid

别名:

α-淀粉酶, PPA, al1,4 葡聚糖-4-葡萄糖水解酶，, 猪胎盘β-N-乙酰葡糖胺糖苷酶, 猪胰腺α-淀粉酶

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About This Item

EC 号:
3.2.1.1 (BRENDA, IUBMB)
EC 号:
232-565-6
MDL编号:
MFCD00081319
UNSPSC代码:
12352204
eCl@ss:
32160410
NACRES:
NA.54
价格与库存信息目前不能提供

生物来源

Porcine pancreas

质量水平

200

类型

Type VI-B

表单

powder

比活

≥5 units/mg solid

环保替代产品特性

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

浓度

~90% (lactose)

技术

activity assay: suitable

适用性

suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification

应用

life science and biopharma

环保替代产品分类

Enabling,

储存温度

2-8°C

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相关类别

一般描述

分子质量：51-54 kDa。
从猪胰腺中分离的α-淀粉酶是一种糖蛋白。它是由约475个残基组成的一条多肽链（包含2个SH基团和4个二硫键）以及一个紧密结合的Ca2+ （稳定性所必需的）组成的。氯离子是活性和稳定性所必需的。活性pH范围为5.5至8.0，最适pH为7。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品针对淀粉乙醇研究进行了优化，可提高能效并防止浪费。有关详细信息，请参阅《Biofiles》的文章。

应用

α-淀粉酶用于水解α-连接多糖的α键，如淀粉和糖原。 Sigma的α-淀粉酶已被用于各种植物研究，如 拟南芥 的代谢研究 [1]。 猪胰腺α-淀粉酶在研究番茄的淀粉代谢和成熟过程中被用于去除淀粉 [2]。

生化/生理作用

α-淀粉酶可水解由三个或多个α-(1,4)连接D-葡萄糖单元组成的多糖的α-(1,4)葡聚糖键。 淀粉和糖原等天然底物被分解成葡萄糖和麦芽糖。 猪胰腺α-淀粉酶是一种糖蛋白，由约475个残基组成的一条多肽链（包含2个SH基团和4个二硫键）以及一个紧密结合的Ca2+ （稳定性所必需的）组成。 最适pH为7。

包装

包装规格取决于α-淀粉酶活性

质量

含乳糖

单位定义

在pH 6.9、温度20 °C条件下，一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。

其他说明

有关复合碳水化合物分析 酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000434128
0000434122
0000434122
0000434128
0000416652

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Amir Amini Khoozani et al.
Foods (Basel, Switzerland), 9(2) (2020-02-09)
The use of Whole Green Banana Flour (WGBF) in bread production may be a strategy to improve the nutritional profile of bread, but the extent of improvement may depend on the processing conditions of the flour. Therefore, WGBF was produced
Massimiliano Carciofi et al.
BMC plant biology, 12, 223-223 (2012-11-23)
Starch is stored in higher plants as granules composed of semi-crystalline amylopectin and amorphous amylose. Starch granules provide energy for the plant during dark periods and for germination of seeds and tubers. Dietary starch is also a highly glycemic carbohydrate
Maria R Romero-Lopez et al.
International journal of molecular sciences, 12(4), 2174-2186 (2011-07-07)
Orange is a tropical fruit used in the juice industry, yielding important quantities of by products. The objective of this work was to obtain a dietary fiber-rich orange bagasse product (DFROBP), evaluate its chemical composition and its use in the
Seyadeh Narges Mazloomi et al.
Foods (Basel, Switzerland), 9(9) (2020-09-06)
In this study, orange seed proteins were hydrolyzed by Alcalase enzyme at different enzyme concentrations 1-3% (v/w) and hydrolysis times (2-5 h), to obtain bioactive peptides showing antioxidant, Angiotensin-converting enzyme (ACE) -inhibitory, and hypoglycemic activities. The highest biological activities (p
Stefan G Pierzynowski et al.
Scientific reports, 7(1), 8628-8628 (2017-08-19)
The studies presented were designed to highlight the impact of pancreatic enzymes on glycemic control and insulin response. Blood glucose and plasma insulin levels were monitored after intravenous, oral or direct gut glucose tolerance tests (GTT) in 6 pigs with
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实验方案

α-淀粉酶酶促测定 (EC 3.2.1.1)

可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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Questions

1–4 of 4 Questions  

  1. The product is insoluble in 0.05 M Phosphate buffer and water. What is the solubility of the product

    1 answer

    1. This product is not specifically tested for solubility. However, as mentioned on the Product Datasheet, the material is expected to be soluble at 0.5 mg/mL in 20mM phosphate-buffered saline containing 6mM Sodium Chloride, pH 6.9. This information is according to the following reference:
      Irondi, F.A. et al., Food Sci. Nutr., 5(2), 233-242 (2017).

      For analytical purposes, this enzyme is prepared at 1 unit/mL in sold deionized water in the determination of enzymatic activity.

      Please see the link below to review the product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/966/306/a3176pis.pdf

      Helpful?

  2. What type of amylase will work effectively with Benedicts reagents for a digestion experiment?

    1 answer

    1. The alpha amylase products have not been tested with Benedict's Reagent to confirm the release of various reducing sugars resulting from starch digestion.

      Helpful?

  3. Could you please provide the approximate weight in grams of the product A3176-500KU?

    1 answer

    1. The product with code A3176-500KU has an approximate weight of 5 grams. This is calculated as follows: 50000 x 1mg / 10 units = 5000mg, and then 5000mg x 1g / 1000mg = 5g.

      Helpful?

  4. What is the optimal temperature for this enzyme?

    1 answer

    1. The enzymatic activity testing of this product is performed at room temperature. This enzyme has a broad temperature range with various publications citing activity from 10 - 80°C, at neutral pH. Please see the links below to review the product datasheet, the enzyme assay protocol, and a sample Certificate of Analysis for additional information:

      Product datasheet
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/966/306/a3176pis.pdf

      Product enzyme assay
      https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-a-amylase

      Sample Certificate of Analysis
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A3176-BULKSLCQ1770.pdf

      Helpful?

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