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Merck

A4862

Sigma-Aldrich

α-淀粉酶 来源于芽孢杆菌

liquid

别名:

Termamyl ® 300L

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54
价格与库存信息目前不能提供

生物来源

Bacillus sp.

表单

liquid

储存温度

2-8°C

相关类别

一般描述

α-淀粉酶属于糖基水解酶家族 13。[1]由 A、B 和 C 结构域组成。结构域 A 有两个天冬氨酸和一个谷氨酸作为催化残基。结构域 B 具有 Ca 2 + 和 Na + 结合位点。[2]

应用

α-淀粉酶来自 芽孢杆菌 的淀粉酶。已用于冻干微藻样品的消化。[3]也用于评价 α-海洋藻类样品[4]Myrmecodia platytyrea中的淀粉酶抑制活性。[5]

生化/生理作用

α-淀粉酶将 α-d-(1,4)-葡糖苷键上的多糖淀粉、麦芽低聚糖水解成α-异头产物。[1]在工业上有广泛的应用,包括 食品、药品、纺织品和纸张。[6]

单位定义

在pH 6.9、20°C下,一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。

法律信息

Novozyme Corp.的产品。
Termamyl is a registered trademark of Novozymes Corp.

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Microalgae Characterization for Consolidated and New Application in Human Food, Animal Feed and Nutraceuticals
Molino A, et al.
International Journal of Environmental Research and Public Health, 15(11), 2436-2436 (2018)
Improving the reversibility of thermal denaturation and catalytic efficiency of Bacillus licheniformis alpha-amylase through stabilizing a long loop in domain B
Li Z, et al.
PLoS ONE, 12(3), e0173187-e0173187 (2017)
Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose
Kagawa M, et al.
Journal of Bacteriology, 185(23), 6981-6984 (2003)
High-performance thin-layer chromatographic methods in the evaluation of the antioxidant and anti-hyperglycemic activity of Myrmecodia platytyrea as a promising opportunity in diabetes treatment
Agatonovic-Kustrin S, et al.
Journal of Chromatography A, 1530, 192-196 (2017)
Snezana Agatonovic-Kustrin et al.
Marine drugs, 17(3) (2019-03-06)
Marine organisms produce an array of biologically active natural products, many of which have unique structures that have not been found in terrestrial organisms. Hence, marine algae provide a unique source of bioactive compounds. The present study investigated 19 marine

实验方案

可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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Questions

  1. Can I dilute the alpha amylase (A4862) in distilled/deionized water or should I use any specific buffer for dilution?

    1 answer
    1. This product may be diluted further using ultrapure water. Please see the link below to review the enzymatic assay method for this product:
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-a-amylase

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