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Merck
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文件

B6149

Sigma-Aldrich

5-溴-4-氯-3-吲哚磷酸 二钠盐

chromogenic, ≥98% (HPLC), powder

别名:

BCIP®, X-磷酸盐 二钠盐

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About This Item

线性分子式:
C8H4BrClNO4P · 2Na
CAS号:
102185-33-1
分子量:
370.43
MDL號碼:
MFCD00036757
分類程式碼代碼:
12352204
PubChem物質ID:
24891897
NACRES:
NA.83

product name

5-溴-4-氯-3-吲哚磷酸 二钠盐, ≥98% (HPLC)

化驗

≥98% (HPLC)

形狀

powder

溶解度

H2O: 20 mg/mL
DMF: insoluble

儲存溫度

−20°C

SMILES 字串

[Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12

InChI

1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2

InChI 密鑰

OAZUOCJOEUNDEK-UHFFFAOYSA-L

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應用

5-溴-4-氯-3-吲哚磷酸二钠盐已用于检测蛋白-抗体复合物。还用于碱性磷酸酶组化分析的组织染色。
用于碱性磷酸酶标记分子比色检测的有 5-溴-4-氯-3-吲哚磷酸 (BCIP) 和硝基四氮唑蓝 (NBT)。BCIP/NBT 底物系统功能多样，可用于多种应用，包括 Northern、Southern 和 Western 印迹、原位杂交和免疫组化。

BCIP 有两种盐形式：溶于水的二钠盐和溶于二甲基甲酰胺的对甲苯胺形式。这些盐形式可用于制备与 NBT 和反应缓冲液结合形成碱性磷酸酶底物溶液的储备液。该底物系统与碱性磷酸酶共同孵育时，会产生一种不溶性的 NBT 二甲苯胺产物，该产物的紫色易于观察。为方便起见，BCIP 和 NBT 的混合物以易于溶解的片剂形式或即用型液体形式提供。

基底

碱性磷酸酶的组织化学底物。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Manli Qi et al.
Journal of bacteriology, 193(10), 2498-2509 (2011-03-29)
The Chlamydia-specific hypothetical protein CT795 was dominantly recognized by human antisera produced during C. trachomatis infection but not by animal antisera raised against dead chlamydia organisms. The immundominant region recognized by the human antibodies was mapped to the N-terminal fragment
P E Høyer et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 44(8), 855-863 (1996-08-01)
We describe a quantitative histochemical method for demonstration of five N-acetyl-glucosamine binding lectins in the syncytiotrophoblast of human term placenta. The method employs biotinylated lectins and alkaline phosphatase-conjugated avidin. The alkaline phosphatase activity is detected by using 5-bromo-4-chloro-indoxyl phosphate as
Shailesh Kumar et al.
PLoS genetics, 15(6), e1008219-e1008219 (2019-06-27)
Fes1 is a conserved armadillo repeat-containing Hsp70 nucleotide exchange factor important for growth at high temperature, proteasomal protein degradation and prion propagation. Depleting or mutating Fes1 induces a stress response and causes defects in these processes that are ascribed solely
Thermal preference ranges correlate with stable signals of universal stress markers in Lake Baikal endemic and Holarctic amphipods
Axenov-Gribanov D, et al
PLoS ONE, 11(10), e0164226-e0164226 (2016)
The endosomal protein endotubin is required for enterocyte differentiation
Cox CM, et al.
Cellular and molecular gastroenterology and hepatology, 5(2), 145-156 (2018)
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比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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