推荐产品
一般描述
昆虫GeneJuice<TMSYMBOL></TMSYMBOL>转染试剂是一种专有的脂质体配方，可优化Sf9昆虫细胞的最大转染效率。该试剂对细胞的毒性极低，可用于在含血清或无血清培养基中进行瞬时和稳定转染，以及将转移质粒与线性化病毒DNA共转染以生产重组杆状病毒。当使用包含立即早期杆状病毒启动子IE1的pIEx<TMSYMBOL></TMSYMBOL>和pBiEx<TMSYMBOL></TMSYMBOL>载体时，昆虫GeneJuice是Sf9昆虫细胞悬浮培养转染中大规模表达蛋白质的理想选择。
昆虫GeneJuice转染试剂以2 mg/ml混悬液的形式在20 mM MES，150 mM NaCl，pH 6.2缓冲液中提供。1 ml足以分别在10 ml悬浮培养瓶或35 mm平板中进行12或125次转染。
- 当昆虫Genejuice®与Sf9细胞系结合使用时，来自海肾荧光素酶表达与plex的原始相对光单位(RLU)为131.90，而其他试剂与Sf9一起使用时为75.726，因此相差1.7倍。
- 当昆虫Genejuice®与Sf9细胞系结合使用时，来自海肾荧光素酶表达与plex的原始相对光单位(RLU)为131.90，而其他试剂与High Five一起使用时为11.952，因此相差11倍。
- 当昆虫Genejuice®与Sf9细胞系结合使用时，来自海肾荧光素酶表达与plex的原始相对光单位(RLU)为131.90，而昆虫Genejuice与High Five一起使用时为31.897，因此相差4.1倍。
昆虫GeneJuice转染试剂以2 mg/ml混悬液的形式在20 mM MES，150 mM NaCl，pH 6.2缓冲液中提供。1 ml足以分别在10 ml悬浮培养瓶或35 mm平板中进行12或125次转染。
警告
毒性：标准处理（A）
法律信息
GeneJuice是EMD Biosciences，Inc.，Madison，WI USA的注册商标
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
其他客户在看
Frauke Hussmann et al.
Scientific reports, 6, 20653-20653 (2016-02-13)
Alp14 is a TOG-family microtubule polymerase from S. pombe that tracks plus ends and accelerates their growth. To interrogate its mechanism, we reconstituted dynamically unstable single isoform S. pombe microtubules with full length Alp14/TOG and Alp7, the TACC-family binding partner
Ylva C Strandberg Lutzow et al.
BMC veterinary research, 4, 18-18 (2008-06-03)
Mastitis in dairy cattle results from infection of mammary tissue by a range of micro-organisms but principally coliform bacteria and Gram positive bacteria such as Staphylococcus aureus. The former species are often acquired by environmental contamination while S. aureus is
Chigang Chen et al.
PloS one, 8(9), e73912-e73912 (2013-09-17)
Xanthine oxidoreductase (XOR) is a cytoplasmic molybdenum-containing oxidoreductase, catalyzing both endogenous purines and exogenous compounds. It is suggested that XOR in porcine hepatocytes catalyzes the N-oxide reduction of quinoxaline 1,4-di-N-oxides (QdNOs). To elucidate the molecular mechanism underlying this metabolism, the
Sandra Sacre et al.
European journal of immunology, 46(3), 772-781 (2015-11-26)
Toll-like receptors (TLRs) are innate immune receptors that respond to both exogenous and endogenous stimuli and are suggested to contribute to the perpetuation of chronic inflammation associated with rheumatoid arthritis (RA). In particular, the endosomal TLRs 3, 7, 8, and
Banzragch Battur et al.
PloS one, 4(9), e7136-e7136 (2009-09-24)
Lysine-ketoglutarate reductase/saccharopine dehydrogenase (LKR/SDH) is a bifunctional enzyme catalyzing the first two steps of lysine catabolism in plants and mammals. However, to date, the properties of the lysine degradation pathway and biological functions of LKR/SDH have been very little described
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门