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Merck
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主要文件

L3287

Sigma-Aldrich

Escort IV 转染试剂

Lipid reagent for transient and stable transfection of mammalian and insect cells.

别名:

Gene delivery

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About This Item

UNSPSC代码:
41106502
NACRES:
NA.25

等级

Molecular Biology
for molecular biology

质量水平

表单

liquid (aqueous solution)

用途

 mL sufficient for 160-500 transfections

浓度

1 mg/mL

技术

transfection: suitable

储存温度

2-8°C

一般描述

Escort IV是由专利的聚阳离子脂质体和中性非转染脂质体构成的独特配方。这种脂质体构成的化合物可用于向不同类型的真核细胞转染核酸。

应用

适用于将核酸瞬时和稳定转染到培养的真核细胞中。 每 6 cm 细胞培养板使用约 4-16μL Escort IV 和 2μg DNA。方案优化提供了非常高效的转染。关于使用 Escort IV 成功转染的细胞列表,请参见 转染试剂选择指南

特点和优势

  • 适于稳定和瞬时转染
  • 针对广泛的细胞类型进行了优化
  • 低细胞毒性
  • 兼容含血清和不含血清的转染实验
  • 非常适于 Sf9, Sf21 和S2昆虫细胞

组分

Escort IV 配方:
1 mg/mL溶于水的总脂质体

请注意,Escort IV 中使用的脂质体具体类别属于机密信息。

注意

注意:不要冻存。

原理

在不存在血清条件下,将Escort IV与DNA混合,即可形成稳定的复合物。该复合物性质稳定,可直接加入到细胞培养基中,并在培养基中与细胞膜融合,将DNA释放到细胞质中。注意:复合物的形成受到血清抑制,但一旦形成稳定的复合物,即便存在血清也无妨。

法律信息

Escort is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

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R'ada Massarwa et al.
Developmental cell, 12(4), 557-569 (2007-04-11)
Formation of syncytial muscle fibers involves repeated rounds of cell fusion between growing myotubes and neighboring myoblasts. We have established that Wsp, the Drosophila homolog of the WASp family of microfilament nucleation-promoting factors, is an essential facilitator of myoblast fusion
Victoria J Hammond et al.
The Analyst, 133(1), 71-75 (2007-12-19)
Sol-gel nanoprobes, also known as Photonic Explorer for Bioanalysis with Biologically Localised Embedding (PEBBLE), capable of performing in-vitro intracellular monitoring of reactive oxygen species have been developed using a modified form of 5(6)-carboxyfluorescein diacetate. A sol-gel matrix was selected for
Eva Krieghoff et al.
Journal of cell science, 119(Pt 7), 1453-1463 (2006-03-24)
beta-catenin is the central signalling molecule of the canonical Wnt pathway, where it activates target genes in a complex with LEF/TCF transcription factors in the nucleus. The regulation of beta-catenin activity is thought to occur mainly on the level of
Manveen K Gupta et al.
Antioxidants & redox signaling, 8(5-6), 1081-1093 (2006-06-15)
Cardiac myocytes, upon exposure to increasing doses of norepinephrine (NE), transit from hypertrophic to apoptotic phenotype. Since reactive oxygen species (ROS) generation is attributed to both phenomena, the authors tested whether an elevation in intracellular ROS level causes such transition.
Zev Bryant et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(3), 772-777 (2006-12-22)
Myosin VI supports movement toward the (-) end of actin filaments, despite sharing extensive sequence and structural homology with (+)-end-directed myosins. A class-specific stretch of amino acids inserted between the converter domain and the lever arm was proposed to provide

商品

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

转染是将DNA、RNA或蛋白质引入真核细胞的过程,用于研究和调节基因表达。因此,转染技术和实验方案作为分析工具,有助于表征遗传功能、蛋白质合成、细胞生长和发育。

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

实验方案

The product bulletin providin detailed use protocol for easy DNA transfection.

Product manual provides detailed protocol for easy DNA transfection.

Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.

Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.

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