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408727

Sigma-Aldrich

支化聚乙烯亚胺

average Mw ~25,000 by LS, average Mn ~10,000 by GPC, branched

别名:

氮丙啶，均聚物, PEI

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About This Item

线性分子式:
H(NHCH2CH2)nNH2
CAS号:
9002-98-6
MDL编号:
MFCD00084427
UNSPSC代码:
12162002
PubChem化学物质编号:
24865591
NACRES:
NA.23
价格与库存信息目前不能提供

质量水平

200

表单

viscous liquid

分子量

average Mn ~10,000 by GPC
average Mw ~25,000 by LS

杂质

≤1% water

折射率

n20/D 1.5290

粘度

13,000-18,000(50 °C)

沸点

250 °C (lit.)

密度

1.030 g/mL at 25 °C

SMILES字符串

C1CN1

InChI

1S/C2H5N/c1-2-3-1/h3H,1-2H2

InChI key

NOWKCMXCCJGMRR-UHFFFAOYSA-N

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一般描述

聚乙烯亚胺（PEI）是一种亲水性阳离子聚合物， 被广泛用作非病毒核苷酸转染试剂。支链PEI可以 通过氮丙环的阳离子开环聚合反应合成。PEI基 颗粒也可用作疫苗的助剂。由于其良好的 理化性质，它在许多领域中得到了应用，如 蛋白质的分离纯化、二氧化碳吸收、药物载体、 废水处理和生物标记。

应用

聚乙烯亚胺可用作非病毒合成聚合物载体，用于治疗性核酸的体内输送。 带负电荷的核酸和带正电荷的聚合物主链之间的相互作用导致纳米络合物的形成。这种中性络合物保护被包裹的核酸免于遭受酶的作用，并维持其稳定性直至发生细胞摄取。例如，与人血清白蛋白结合的PEI 具有良好的pDNA 转染性能和低毒性。

PEI可用于单壁纳米管 (SWNT) 的功能化修饰，以改善其溶解性和生物相容性，同时维持原始SWNT的结构完整性。共价功能化的SWNT可应用于 CO2 吸收和基因递送。

支链PEI也可用于改变吸附剂的表面性质。PEI修饰的水合氧化锆/PAN纳米纤维可用于地下水的脱氟，因为其具有高氟吸附能力和宽工作pH范围。

特点和优势

PEI的伯胺和仲胺基团可以高效地结合 药物、核酸和其它功能基团。

支链PEI 具有更好的络合和缓冲能力。

外形

支化聚合物

象形图

Exclamation markEnvironment
GHS07,GHS09

警示用语：

Warning

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 2

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCM8375
BCCM5483
BCCM5482
BCCM2321
STBL4117

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Jessel, N., et al.
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The development of osteochondral tissue engineering is an important issue for the treatment of traumatic injury or aging associated joint disease. However, the different compositions and mechanical properties of cartilage and subchondral bone show the complexity of this tissue interface
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Sequential infection with antigenically distinct influenza viruses induces cross-protective immune responses against heterologous virus strains in animal models. Here we investigated whether sequential immunization with antigenically distinct influenza vaccines can also provide cross-protection. To this end, we compared immune responses
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Questions

1–10 of 23 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Do you have any GPC (Gel permeation chromatography) data for this product n°408727 that you can share?

    1 answer

    1. This product is not tested by Gel Permeation Chromatography. The molecular weight distribution of this material has not been determined. However, historical information indicates the mole fraction of primary, secondary, and tertiary amines is 1:1.20:0.76. The material dispersity (Mw / Mn) is 25,000 / 10,000 = 2.5.

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  4. Is Branched PEI soluble in Diethylene glycol and other glycols? Do these solvents affect the amine functions/charges?

    1 answer

    1. This compound is readily soluble in hot water and some organic solvents, such as methanol, ethanol, or chloroform. However, the solubility in diethylene glycol or other glycols has not been determined.

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  5. Can this product be used for stable transfection?

    1 answer

    1. This product is not transfection grade and has not been tested as suitable for this type of application. It is the responsibility of the end user to determine suitability for this type of application. A search of the scientific literature does reveal that a few laboratories have reported the use of this product for transfection. Those article citations can be found below.

      Hoenigsperger, H., Koepke, L., Acharya, D., Hunszinger, V., Freisem, D., Grenzner, A., … Sparrer, K. M. J. (2023). CSNK2 suppresses autophagy by activating FLN-NHL-containing TRIM proteins. Autophagy, 1–21. https://doi.org/10.1080/15548627.2023.2281128

      Kritika Saxena, Nicola Domenico Roverato, Melody Reithmann, Mei Min Mah, Richard Schregle, Gunter Schmidtke, Ivan Silbern, Henning Urlaub, Annette Aichem. (2023) Phosphorylated FAT10 interferes with IFN-I signaling Life Science Alliance DOI: 10.26508/lsa.202101282

      Kirman, D.C., Renganathan, B., Chui, W.K. et al. Cell surface nucleolin is a novel ADAMTS5 receptor mediating endothelial cell apoptosis. Cell Death Dis 13, 172 (2022). https://doi.org/10.1038/s41419-022-04618-x

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  6. What recommended storage conditions (temperature) of PEI? Is refrigeration or freezing suitable? And how shelf-life at storage conditions? Impact of dilution on PEI's shelf-life ?

    1 answer

    1. Product no. 408727 Polyethylenimine, branched is stored at ambient temperature and does not have an assigned shelf-life.

      We offered a 2 mg/ml Branched polyethylenimine solution (Product no. 913375) that was stable for 2 years at 2 - 8 degrees C.

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  7. Can you please provide me with the number of primary amines per molecule for the product with the code 408727?

    1 answer

    1. Approximately 6.4 mmol of primary amines per gram of dry substance. This calculation is derived from the equation 1/(1+1.1+0.7) multiplied by 18, resulting in 6.4.

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  8. Is this polymer soluble in THF?

    1 answer

    1. The solubility of this product is not tested on a lot-to-lot basis. This compound is readily soluble in water and sparingly soluble in organic solvents such as DMSO and acetone. Other sources report that this material may also be sparingly soluble in THF. This information has not been validated.

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  9. What is the N(Nitrogen)% for following PEI? Polyethylenimine, branched, average Mw ~25,000 by LS, average Mn ~10,000 by GPC, branched, 250ml

    1 answer

    1. Unfortunately, the N content of Product 408727 is not determined.

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  10. What is the concentration of 408727-100ML in mg/ml?

    1 answer

    1. This product is a 'neat' or pure liquid. The density is 1.03 g/ml or 1030 mg/ml. The molarity of the material is 41.2 mM, based on average Mw ~25,000 by LS and the previously mentioned density.

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