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Merck
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文件

P5726

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 2

aqueous solution (dark coloration may develop upon storage, which does not affect the activity), for the inhibition of tyrosine protein phosphatases, acid and alkaline phosphatases

别名:

Protease inhibitor

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.54

product name

磷酸酶抑制剂混合物2, aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

形狀

aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

品質等級

300

運輸包裝

wet ice

儲存溫度

2-8°C

相关类别

一般說明

磷酸酶 抑制剂混合液2是一种成分混合物，旨在抑制 修饰细胞提取物中蛋白质的酶，从而提高完整蛋白质 产量。它已经过优化和测试，适用于各种动物 组织和细胞质提取物。

應用

磷酸酶抑制剂混合物可通过向能够对细胞提取物中存在的蛋白进行修饰的酶添加抑制剂来提高完整蛋白的产量。 该产品已针对酪氨酸蛋白磷酸酶、酸性和碱性磷酸酶进行了优化和测试。 它已对来自包括牛肝和人胎盘的动物组织的细胞提取物和兔肌肉细胞提取物进行了测试。

生化／生理作用

该混合物含有具有特异性抑制属性的独立成分。 原钒酸钠可抑制多种ATP酶、蛋白酪氨酸磷酸酶和其他磷酸转移酶。 钼酸钠可抑制酸性和磷蛋白磷酸酶。 酒石酸钠可抑制酸性磷酸酶。 咪唑可抑制碱性磷酸酶。

特點和優勢

该化合物是激酶磷酸酶生物学研究的特色产品。点击此处以发现更多的激酶磷酸酶生物学研究特色产品。可访问sigma.com/discover-bsm了解更多关于用于其他研究领域生物活性小分子。

注意

该产品在供货后可在推荐的2-8°C储存温度下稳定两年。

準備報告

该混合物以透明溶液形式提供。 它已通过无菌填充的瓶子中的0.2 μm膜进行了无菌过滤。 一毫升将可抑制来自人胎盘、牛肝、兔肌肉、A431或Jurkat细胞提取物的100,000 x g上清液中发现的蛋白浓度约为5 mg/mL的磷酸酶活性。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

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产品编号
说明
价格

相關產品

产品编号
说明
价格

象形圖

Health hazardExclamation mark
GHS08,GHS07

訊號詞

Danger

危險聲明

H315,H319,H360D

危險分類

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 2

分析证书(COA)

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Colin S McCoin et al.
American journal of physiology. Endocrinology and metabolism, 317(2), E298-E311 (2019-05-01)
The impact of sexual dimorphism and mitophagy on hepatic mitochondrial adaptations during the treatment of steatosis with physical activity are largely unknown. Here, we tested if deficiencies in liver-specific peroxisome proliferative activated-receptor-γ coactivator-1α (PGC-1α), a transcriptional coactivator of biogenesis, and
Blanca Majem et al.
Oncogene, 38(32), 6035-6050 (2019-07-07)
Ovarian cancer is the most lethal gynecological malignancy due to the silent nature on its early onset and the rapid acquisition of drug resistance. Histologically heterogeneous, it includes several subtypes with different mutational landscapes, hampering the development of effective targeted
Shira Simonovitch et al.
Journal of Alzheimer's disease : JAD, 70(3), 861-875 (2019-07-16)
This study examined the effects of apolipoprotein E4 (APOE4), the most prevalent genetic risk factor for Alzheimer's disease (AD), on proteins involved in mitochondrial dynamics and autophagy, in the hippocampus of targeted replacement mice. Immunohistochemical measurements revealed that the levels
Uchenna John Unachukwu et al.
Scientific reports, 6, 22392-22392 (2016-03-05)
To replace photoreceptors lost to disease or trauma and restore vision, laboratories around the world are investigating photoreceptor replacement strategies using subretinal transplantation of photoreceptor precursor cells (PPCs) and retinal progenitor cells (RPCs). Significant obstacles to advancement of photoreceptor cell-replacement
G I Lancaster et al.
Nature communications, 7, 10626-10626 (2016-02-04)
Protein kinase R (PKR) has previously been suggested to mediate many of the deleterious consequences of a high-fat diet (HFD). However, previous studies have observed substantial phenotypic variability when examining the metabolic consequences of PKR deletion. Accordingly, herein, we have
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Phosphatase Inhibitor Cocktail

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磷酸酶抑制剂混合物

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