P4762

Papain from papaya latex

lyophilized powder, ≥10 units/mg protein

• 동의어(들): Papainase
• CAS Number: 9001-73-4
• 효소 위원회 번호: 3.4.22.2 (BRENDA, IUBMB)
• EC Number: 232-627-2
• MDL number: MFCD00131791
• UNSPSC 코드: 12352204
• eCl@ss: 42030311
• NACRES: NA.54

속성

• 생물학적 소스: papaya (latex)
• 양식: lyophilized powder
• 특이 활성도: ≥10 units/mg protein
• 분자량: 23.4 kDa
• 기술: cell culture | mammalian: suitable; single cell analysis: suitable
• 응용 분야: diagnostic assay manufacturing
• 저장 온도: −20°C

설명

일반 설명

Papain is a cysteine protease, found in Carica papaya. It is composed of a single polypeptide chain containing three disulfide bridges and a sulfhydryl group.[1]

애플리케이션

Papain from papaya latex has been used:

• to digest carotid artery smooth muscle cells[2]
• in dissecting solutions
• to produce Fab fragments of antibodies[3]
• for cell dissociation since it has been shown to be more effective and less damaging with certain tissues[4][5]
• in the enzyme cocktail for dissociating human fetal retinal tissue[6]
• to digest chondrocytes/scaffold cultures for total DNA quantification for cell proliferation[7]
• as a supplement in neurobasal medium to produce retinal cell suspensions[8]

Suitable for use in preparation of single cell suspension for sequencing.

Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

생화학적/생리학적 작용

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Papain with wide specificity,[9] cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. Papain is considered to digest protein substrates more broadly than the pancreatic proteases.[10] Papain is commonly used in cell isolation procedures as it is highly efficient and less harmful than other proteases on certain tissues.[11] The use of papain is known to yield more viable smooth muscle cells, retaining the cell′s sensitivity to stimulants.[12] Papain papaya latex has antifungal activity against C. albicans and is a cysteine protease.

• The pH optimum is 6.0-7.0
• Papain hydrolyzes esters and amides

포장

Package size based on protein content

단위 정의

One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.

물리적 형태

Lyophilized powder containing sodium acetate

제조 메모

2× Crystallized

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves