76216

Papain from Carica papaya

solution, light brown, ≥10 U/mg protein (~25 mg/ml)

• CAS Number: 9001-73-4
• 효소 위원회 번호: 3.4.22.2 (BRENDA, IUBMB)
• EC Number: 232-627-2
• MDL number: MFCD00131791
• UNSPSC 코드: 12352204
• eCl@ss: 42030311
• NACRES: NA.54

속성

• 생물학적 소스: papaya
• Quality Level: 100
• 양식: solution
• 특이 활성도: ≥10 U/mg protein (~25 mg/ml)
• 분자량: M_r ~23000
• 기술: cell culture | mammalian: suitable; single cell analysis: suitable
• 색상: light brown
• 저장 온도: 2-8°C

설명

일반 설명

Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.[1]

애플리케이션

Papain from Carica papaya has been used:

• in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture[2]
• to study its effects on Alpaca seminal plasma viscosity and sperm function[3]
• to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa[4]

Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.

생화학적/생리학적 작용

Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.

Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food[1], baking, and dairy industries.[5]

단위 정의

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves