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추천 제품
양식
lyophilized powder
Quality Level
기술
DNA extraction: suitable
적합성
suitable for microbiology
응용 분야
microbiology
배송 상태
wet ice
저장 온도
−20°C
일반 설명
Metagenomics analysis looks at all DNA that has been isolated directly from given single samples (e.g. environmental samples, biological organisms). Metagenomics allows for the investigation of microbes that exist in any environment (including extreme environments), and which have been historically difficult to isolate, culture, and study. Metagenomics has revealed the existence of novel microbial species. Applications of metagenomic studies include public health data analysis, discovery of novel proteins, enzymes and natural products, environmental studies, and agricultural investigations.
애플리케이션
MetaPolyzyme is a mixture of 6 enzymes that is intended for isolation of total DNA for metagenomics studies. Adapted from an initial formulation devised by S. Tighe,2 MetaPolyzyme was evaluated and developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG).[1] Test data on the use of MetaPolyzyme is accessible at the 2017 ABRF MGRG Poster.
생화학적/생리학적 작용
Microbes can be difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted by using lysing enzymes (such as lyticase or chitinase) to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. MetaPolyzyme is intended for use in metagenomic studies, such as study of microbes and microbiomes of extreme and unique environments. MetaPolyzyme is intended for digestion of microbes for evaluation by whole genome shotgun sequencing for metagenomics and metatranscriptomic approaches.
성분
The enzymes in MetaPolyzme are:
- Achromopeptidase
- Chitinase
- Lyticase
- Lysostaphin
- Lysozyme
- Mutanolysin
단위 정의
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
이미 열람한 고객
The Metagenomics and Metadesign of the Subways and Urban Biomes (MetaSUB) International Consortium inaugural meeting report.
MetaSUB International Consortium
Microbiome, 4(1), 24-24 (2016)
Genomic Methods and Microbiological Technologies for Profiling Novel and Extreme Environments for the Extreme Microbiome Project (XMP).
Tighe S et al.
Journal of biomolecular techniques : JBT, 28(1), 31-39 (2017)
Microbiome, 4(1), 24-24 (2016-06-04)
The Metagenomics and Metadesign of the Subways and Urban Biomes (MetaSUB) International Consortium is a novel, interdisciplinary initiative comprised of experts across many fields, including genomics, data analysis, engineering, public health, and architecture. The ultimate goal of the MetaSUB Consortium
Metagenome sequencing-based strain-level and functional characterization of supragingival microbiome associated with dental caries in children.
Al-Hebshi NN et al.
Journal of Oral Microbiology, 11(1), 1557986-1557986 (2018)
Microbiomic Analysis on Low Abundant Respiratory Biomass Samples; Improved Recovery of Microbial DNA From Bronchoalveolar Lavage Fluid.
Saladie M et al.
Frontiers in Microbiology, 11, 572504-572504 (2020)
문서
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
세포 용해 효소와 원형질체 준비 공정을 명확히 설명하여 시료 준비 중 발생하는 복잡한 효소 반응을 자세히 나타냅니다.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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