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Merck
모든 사진(2)

주요 문서

SAE0091

Sigma-Aldrich

Lysostaphin from Staphylococcus staphylolyticus

free of DNA contaminants, ≥500 units/mg protein, lyophilized powder, suitable for Microbiome research

동의어(들):

Lysostaphin from Staphylococcus staphylolyticus, Glycyl-glycine Endopeptidase

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크기 선택

2 MG
₩342,055

₩342,055


출고 가능일2025년 4월 16일세부사항


벌크 견적 요청

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2 MG
₩342,055

About This Item

CAS Number:
효소 위원회 번호:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.54

₩342,055


출고 가능일2025년 4월 16일세부사항


벌크 견적 요청

제품명

Lysostaphin from Staphylococcus staphylolyticus, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein

생물학적 소스

microbial (Staphylococcus staphylotyticus)

양식

lyophilized powder

특이 활성도

≥500 units/mg protein

분자량

27 kDa

특징

DNA free

배송 상태

wet ice

저장 온도

−20°C

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애플리케이션

Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)

생화학적/생리학적 작용

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5
Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.

단위 정의

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

물리적 형태

Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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Anna Cäcilia Ingham et al.
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Judy J J Ou et al.
Frontiers in cellular and infection microbiology, 6, 187-187 (2017-01-14)
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문서

Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.

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