추천 제품
Quality Level
형태
lyophilized powder
특징
DNA free
기술
DNA extraction: suitable
적합성
suitable for microbiology
응용 분야
microbiology
배송 상태
wet ice
저장 온도
−20°C
관련 카테고리
일반 설명
Metagenomics analysis looks at all DNA that has been isolated directly from given single samples (e.g. environmental samples, biological organisms). Metagenomics allows for the investigation of microbes that exist in any environment (including extreme environments), and which have been historically difficult to isolate, culture, and study. Metagenomics has revealed the existence of novel microbial species. Applications of metagenomic studies include public health data analysis, discovery of novel proteins, enzymes and natural products, environmental studies, and agricultural investigations.
MetaPolyzyme products (MAC4L and MAC4LDF) are based on a multi-lytic enzyme mixture formulated for effective lysis of Microbiome samples from extreme environments. Originally developed by Scott Tighe for use in microbiome and DNA extraction efficiency studies, the products were evaluated and developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG; formerly the Metagenomics Research Group, MGRG).1-4 Several publications have cited use of MetaPolyzyme.5-7
MetaPolyzyme products (MAC4L and MAC4LDF) are based on a multi-lytic enzyme mixture formulated for effective lysis of Microbiome samples from extreme environments. Originally developed by Scott Tighe for use in microbiome and DNA extraction efficiency studies, the products were evaluated and developed in consultation and collaboration with the Association of Biomolecular Resource Facilities (ABRF) Metagenomics and Microbiome Research Group (MMRG; formerly the Metagenomics Research Group, MGRG).1-4 Several publications have cited use of MetaPolyzyme.5-7
애플리케이션
Our experts have shown that using MetaPolyzyme, DNA free in a microbial study can increase final DNA concentration by approximately 2X, and increase the number of taxa identified. It is useful for low biomass samples for lysing cells without contributing to DNA background contamination. Learn more about the experiments and data by reviewing our technical article – MetaPolyzyme, DNA Free Cell Lysis Enzymes for Microbiome Workflows.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. MetaPolyzyme, DNA free, undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
The need for DNA free reagents led to the development of MetaPolyzyme, DNA free, which is essential when there is a need to minimize microbial DNA contamination from reagents during microbiome studies.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. MetaPolyzyme, DNA free, undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
The need for DNA free reagents led to the development of MetaPolyzyme, DNA free, which is essential when there is a need to minimize microbial DNA contamination from reagents during microbiome studies.
특징 및 장점
Multi-lytic enzyme mixture designed for effective lysis of microbiome samples
DNA-free formulation minimizes contamination
Ideal for low biomass samples
Strict quality control testing ensures absence of detectable levels of contaminating microbial DNA
Increases final DNA concentration and number of taxa identified
DNA-free formulation minimizes contamination
Ideal for low biomass samples
Strict quality control testing ensures absence of detectable levels of contaminating microbial DNA
Increases final DNA concentration and number of taxa identified
성분
The enzymes in MetaPolzyme, DNA free are:
All the enzymes are individually tested for absence of contaminating microbial DNA using 16S and 18S PCR amplification
- Mutanolysin
- Achromopeptidase
- Chitinase
- Lyticase
- Lysostaphin
- Lysozyme
All the enzymes are individually tested for absence of contaminating microbial DNA using 16S and 18S PCR amplification
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
Explore the benefits of MetaPolyzyme, DNA free to improve DNA extraction and increase the number of taxa identified in treated samples.
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.