추천 제품
Quality Level
무균
sterile-filtered
양식
liquid
기술
cell culture | mammalian: suitable
불순물
endotoxin, tested
성분
L-glutamine: no
배송 상태
ambient
저장 온도
2-8°C
일반 설명
MegaCell™ is a line of versatile media, formulated to significantly reduce the amount of serum required for cultivating mammalian cells in vitro. It is effective as a basal formulation for the growth and maintenance of adherent or suspension cell lines. When supplemented with 3% fetal bovine serum (FBS), MegaCell supports proliferative rates and maximal cell densities comparable, and in some cases superior, to the conventional basal formulation supplemented with 10% fetal bovine serum.
애플리케이션
MegaCell™ RPMI-1640 Medium has been used:
- as a component of the TSC medium for culturing trophoblast stem cells (TSC)[1]
- as a component of 25 ng/ml FGF4 and 1 g/ml heparin (TSF4H medium) for culturing TSCs[2]
- as a component of embryo-like structures (ETS) embryo medium and TS cell medium for culturing embryonic stem cells (ESCs) and TSCs[3]
재구성
Supplement with 4 mM L-glutamine (20 ml of 200 mM solution, G7513)
법적 정보
Sigma-Aldrich uses the MegaCell trademark pursuant to an agreement with Promega Corporation.
MegaCell is a trademark of Promega Corp.
또한 이 제품과 함께 일반적으로 구입
제품 번호
설명
가격
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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이미 열람한 고객
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Nature protocols, 13(7), 1586-1602 (2018-07-11)
Mammalian embryogenesis requires the coordination of embryonic and extra-embryonic tissues to enable implantation into the uterus and post-implantation development to establish the body plan. Mouse embryonic stem cells (ESCs) are a useful tool for studying pluripotent embryonic tissue in vitro.
Nina S Corsini et al.
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Mammalian blastocysts comprise three distinct cell lineages essential for development beyond implantation: the pluripotent epiblast, which generates the future embryo, and surrounding it the extra-embryonic primitive endoderm and the trophectoderm tissues. Embryonic stem cells can reintegrate into embryogenesis but contribute
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Stem cell transplantation is a promising potential therapy for muscular dystrophies, but for this purpose, the cells need to be systemically-deliverable, give rise to many muscle fibres and functionally reconstitute the satellite cell niche in the majority of the patient's
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