In general, MEM typically contains 0.292 g/L L-Glutamine. A 200mM Glutamine solution is equivalent to 29.23 mg/mL. So it needs to be diluted around 100 times.
추천 제품
무균
sterile-filtered
Quality Level
300
400
제품 라인
BioXtra
양식
solution
농도
200 mM
기술
cell culture | mammalian: suitable
불순물
endotoxin, tested
배송 상태
dry ice
저장 온도
−20°C
SMILES string
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
유전자 정보
rat ... Ggt1(116568)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
애플리케이션
제조 메모
관련 제품
애플리케이션
추천
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
이미 열람한 고객
문서
Importance and uses of glutamine in hybridoma and mammalian cell culture
Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. We have generated transporter knockout (KO) cell lines using CompoZr® Zinc Finger Nuclease (ZFN) technology in a proprietary renal proximal tubule epithelial cell line.
Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.
Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.
프로토콜
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
Chromatograms
application for HPLCapplication for HPLC-
How much of Product G7513 should be used in cell culture media?
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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
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Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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Why is there a precipitate in the 200 mM L-glutamine solution, products G7513 and G2150, after thawing at room temperature?
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L-Glutamine, 200 mM solution, tends to precipitate in solution when chilled. After thawing, it will be an opalescent solution. If allowed to settle, there can be some crystalline insolubles at the bottom of the bottle.The product should be warmed to 37°C for complete solubilization. To limit the number of times the product needs to be warmed to 37°C, the product can be divided into working aliquots which are stored at -20°C and used as needed. This will expose the product to the minimal amount of warming cycles.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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