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F2442

Sigma-Aldrich

Fetal Bovine Serum

Fetal Bovine Serum
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USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

동의어(들):

Fetal Bovine Sera, Fetal Calf Serum, FBS, FCS, sera, serum

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크기 선택

100 ML
₩246,036
500 ML
₩1,140,048

₩246,036

구입 가능 여부는 고객센터에 문의하십시오.
벌크 견적 요청시료 샘플 요청
모든 사진(1)

크기 선택

보기 변경
100 ML
₩246,036
500 ML
₩1,140,048

About This Item

MDL number:
MFCD00132239
UNSPSC 코드:
12352207
eCl@ss:
32160409
NACRES:
NA.71
생물학적 소스:
bovine fetus
기원:
USA origin
무균:
sterile-filtered
배송 상태:
dry ice

₩246,036

구입 가능 여부는 고객센터에 문의하십시오.
벌크 견적 요청시료 샘플 요청

생물학적 소스

bovine fetus

Quality Level

500

무균

sterile-filtered

제품 라인

FBS Classic

구성

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

기원

USA origin

기술

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

불순물

≤10 EU/mL endotoxin

배송 상태

dry ice

저장 온도

−20°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting optimal growth-enhancing and growth-suppressing functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media.[1][2] FBS is derived from the calf blood. It is devoid of fibrin and clotting factors. Heat inactivation of FBS blocks the complement system.[3] FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture.[4][5] It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM),[6] Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM),[7] M199[8] and Roswell Park Memorial Institute medium, RPMI-1640.[6]

애플리케이션

Fetal Bovine Serum has also been used as a component of:
  • perivascular-resident macrophage-like melanocytes (PVM/M) growth medium[9]
  • synthetic oviduct fluid (SOF)[10]
  • Eagle′s minimum essential medium (EMEM)[11]
  • neurobasal medium[12]
  • Hyclone McCoys 5 A medium[13]
  • Hanks medium[14]

생화학적/생리학적 작용

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

분석 메모

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

기타 정보

Tested for the presence of bacteria, virus, mycoplasma, endotoxin and for hemoglobin content. Triple filtered with 0.1 micron membrane under aseptic conditions.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
19G057-A
24B128
0000321240
23A316
23A317

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

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MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.
Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
Cunzhi Yu et al.
Frontiers in pharmacology, 8, 832-832 (2017-12-07)
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide
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Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

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관련 콘텐츠

Evaluating of Stericup® Quick Release Filtration Devices for Fetal Bovine Serum (FBS) in Cell Culture

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Protocol Guide: Generation of Apical-Out Gastrointestinal Organoids

Step-by-step protocol for generating apical-out human gut organoids for microbiome, ADME/Tox, viral and gastrointestinal related disease research. See the complete organoid culture protocol.

성공으로 이끄는 우태혈청.

머크의 포괄적인 우태혈청(FBS) 포트폴리오에서 귀하의 실험실 요건과 예산에 적합한 제품을 찾아보십시오. 지구촌의 문제가 FBS 가격에 미치는 영향에 대해 알아보십시오.

Cell Culture Workflow

Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

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질문

1–10 / 15 질문  

  1. How to heat inactivate this serum Fetal Bovine Serum USA origin,(Cat Log - F2442)

    1 답변

    1. Heat inactivation occurs when serum is heated to 56°C for a period of 30 minutes.

      Here's a protocol for heat inactivation:
      1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
      2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
      3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
      4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.

      도움이 되었습니까?

  2. is this Heat Inactivate FBS?

    1 답변

    1. This product has not been heat inactivated.

      도움이 되었습니까?

  3. can F2442 FBS be used for culturing peritoneal macrophages? what is the suitable one?

    1 답변

    1. This product is tested for suitability in mammalian cell culture. While it is not specifically tested on macrophages, it is subject to stringent specifications, including low endotoxin, and should perform well. Please see the link below to review a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/coa/SIGMA/F2442/23A317

      도움이 되었습니까?

  4. Can this f2442 fbs be used for culture expansion of mesenchymal stem cells? Please confirm Thanks

    1 답변

    1. Fetal Bovine Serum (Cat. no.: F2442) is suitable for use in mammalian cell culture. However, it is not tested specifically in mesenchymal stem cell culture. There is some evidence in the literature that this product would be suitable. See the link below for a relevant reference:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545441/

      도움이 되었습니까?

  5. What is the difference between Fetal Bovine Serum F2442 and 12103C?

    1 답변

    1. Product No. 12103C meets most of the specifications of F2442.  Both products are from the same raw material suppliers and undergo the same stringent testing prior to release. However, the F2442 has been use-tested in hybridoma culture, whereas 12103C has not.

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  6. There is a precipitate in my serum.  Is my serum contaminated?

    1 답변

    1. Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.

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  7. What is the cloning assay performed on Fetal Bovine Serum, Product F2442?

    1 답변

    1. The cloning efficiency assay is performed on each lot of FBS to verify that each lot will support clonal growth of myeloma cells and/or fusion products. The assay is performed using 5% and 10% concentrations of the FBS.

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  8. How is Fetal Bovine Serum, Product F2442, cell culture tested?

    1 답변

    1. Biological performance is assessed using Sigma cell culture medium supplemented with the serum and tested at a final concentration of 10%. Seeding efficiency, doubling time, and final cell density are measured on appropriate cell lines. Growth studies are carried through two subculture generations and cell growth is plotted as a logarithmic function of time in culture. During the testing period, cultures are examined microscopically for atypical morphology and evidence of cytotoxicity. Hybridoma -tested serum has been shown to support mouse myeloma cells and hybridoma cells in culture.

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  9. Is Fetal Bovine Serum, Product F2442, available as a charcoal stripped, de-lipidated serum?

    1 답변

    1. We offer Product No. F6765 (Fetal Bovine Serum, charcoal stripped). The charcoal stripping decreases the levels of hormones; it may also decrease the levels of lipids. If further reduction of lipid levels is required, you may need to treat the serum with fumed silica (e.g. Product No. S5130). Use 20 grams of S5130 per Liter of serum (not plasma). It is mixed for 3-4 hours, then centrifuged in 1 L containers for about 15 minutes at 2000 xg. The supernatant can then be decanted, and filtered if desired. NOTE: Silica is extremely fluffy, and care must be taken not to inhale its dust. Allow for about 15% loss of the serum due to adsorption.

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  10. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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활성 필터

  1. 대한민국 서울
    • 상품평 1개
    • 투표 4개
    별 5개 중 5개입니다.

    최고의 갓성비 FBS!

    정기배송으로 진행시 가격도 50만원으로 저렴하고 정해진 날짜에 와서 좋아요!
    DMSO같이 많이 쓰는 제품도 media와 같이 묶어서 사용하면 더 저렴해지더라구요 ㅎ

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