An antibody concentration of 1 ug/mL or 1:1000 dilution is recommended for use in western blot. Please see the link below to review the product datasheet which includes the full protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf
F1804
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
동의어(들):
flag antibody, Anti-ddddk, Anti-dykddddk
로그인조직 및 계약 가격 보기
모든 사진(1)
크기 선택
보기 변경
50 μG
₩208,989
200 μG
₩808,854
1 MG
₩1,674,547
5 MG
₩2,694,521
About This Item
UNSPSC 코드:
12352203
NACRES:
NA.32
클론:
M2, monoclonal
application:
종 반응성:
all
citations:
9676
추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
M2, monoclonal
양식
buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
정제법
affinity chromatography (affinity tag purification)
종 반응성
all
농도
1 mg/mL
동형
IgG1
면역원 서열
DYKDDDDK
배송 상태
wet ice
저장 온도
−20°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.
Method of purification - Affinity tag purification
Method of purification - Affinity tag purification
특이성
Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
면역원
FLAG; peptide sequence DYKDDDDK
애플리케이션
For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting[1], immunoprecipitation (IP) [2][3][4], immunohistochemisty, immunofluorescence[5] and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
물리적 형태
Contains 50% glycerol in phosphate buffered saline.
제조 메모
Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk
법적 정보
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
또한 이 제품과 함께 일반적으로 구입
제품 번호
설명
가격
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
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Yalan Liu
Virology, 154-154 (2016)
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Biochimica et biophysica acta. Molecular cell research, 1866(9), 1412-1420 (2019-06-10)
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Michael Seymour
G3 (Bethesda, Md.) (2016)
문서
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
관련 콘텐츠
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
단백질 및 핵산 상호작용 시약과 단백질-RNA, 단백질-DNA, 단백질-단백질 상호작용 및 관련 애플리케이션 투자용 관련 자료
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モノクローナル抗FLAG® M2抗体 マウス宿主抗体(F1804)を購入しました。WBで使用しますが、抗体の推奨の希釈率、希釈液を知りたいです。よろしくお願いいたします。
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