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Merck
모든 사진(6)

주요 문서

CWA-1069

Sigma-Aldrich

Anti-Human PD-L1 (29E.2A3) Colorwheel® Dye-Ready mAb

greener alternative

for use with ColorWheel® Dyes (Required, sold separately)

동의어(들):

Programmed cell death 1 ligand 1

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About This Item

UNSPSC 코드:
41116015
NACRES:
NA.41
제품 CWA-1069은(는)귀하의 국가에서 현재 판매되지 않습니다. 고객지원팀으로 연락바랍니다.

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

29E.2A3, monoclonal

제품 라인

ColorWheel®

양식

lyophilized

분자량

calculated mol wt 33.28 kDa

정제법

using Protein A

종 반응성

human

종 반응성(상동성에 의해 예측)

rat

포장

antibody small pack of 25 μL

환경친화적 대안 제품 특성

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

기술

flow cytometry: suitable

동형

IgG2bκ

에피토프 서열

Extracellular domain

단백질 ID 수납 번호

UniProt 수납 번호

호환성

for use with ColorWheel® Dyes (Required, sold separately)

환경친화적 대안 카테고리

배송 상태

ambient

저장 온도

2-8°C

유전자 정보

mouse ... CD274(29126)

일반 설명

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

특이성

Clone 29E.2A3 is a mouse monoclonal antibody that detects human PD-L1 (CD274). It targets an epitope within the extracellular domain.

면역원

Full-length recombinant human PD-L1.

애플리케이션

Quality Control Testing

Evaluated by Flow cytometry in PHA-stimulated human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): Staining of one million PHA-stimulated Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1069, Anti-Human PD-L1 (29E.2A3) ColorWheel® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG2b isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

호환성

Requires ColorWheel® Dye (Sold Separately)

표적 설명

Programmed cell death 1 ligand 1 (UniProt: Q9NZQ7; also known as PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1, CD274) is encoded by the CD274 (also known as B7H1, PDCD1L1, PDCD1LG1, PDL1) gene (Gene ID: 29126) in human. PD-L1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature form that contains a large extracellular (aa 19-238) with an Ig-like V-type domain (aa 19-127) and an Ig-like C2-type domain (aa 133-225), followed by a transmembrane domain (aa 239-259) and a cytoplasmic tail (aa 260-290). PD-L1 is expressed on activated T- and B-cells, dendritic cells, keratinocytes, and monocytes. Higher expression has also been reported in heart, skeletal muscle, placenta, and lung. It plays a critical role in induction and maintenance of immune tolerance to self. When engaged together with the TCR, the interaction of PD-1 with its ligands, PD-L1 and PD-L2, deliver an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and nonhematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Under some conditions, blockade of this pathway can restore many T cell functions.

물리적 형태

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

재구성

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

법적 정보

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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필요하나 제공되지 않음

제품 번호
설명
가격

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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Lot/Batch Number

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문서 라이브러리 방문

관련 콘텐츠

ColorWheel® 유세포 분석 항체와 염료를 통해 유세포 분석 설계에 자유를 누리십시오. ColorWheel® 유세포 분석 포트폴리오는 품질의 저하 없이 유세포 분석 워크플로를 간소화할 수 있도록 단순화와 유연성을 염두에 두고 설계되었습니다.

질문

후기

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활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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