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주요 문서

MDA-MB-231 Cell Line human

92020424, human breast, Epithelial

동의어(들):

MD Anderson-Metastatic Breast-231 Cells, MDA-MB231 Cells, MDA231-BRE Cells, MDAMB231 Cells

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About This Item

UNSPSC 코드:
41106514
eCl@ss:
32190102
가격 및 재고 정보를 현재 이용할 수 없음

제품명

MDA-MB-231 Cell Line human, human breast adenocarcinoma, 92020424

생물학적 소스

human breast

성장 모드

Adherent

핵형

Modal no.′s 62 and 64, near triploid

형태학

Epithelial

제품

Not specified

수용체

Not specified

기술

cell culture | mammalian: suitable

관련 질환(들)

cancer

배송 상태

dry ice

저장 온도

−196°C

세포주 기원

Human Caucasian breast adenocarcinoma

세포주 설명

Isolated from pleural effusions of a breast cancer patient.

애플리케이션

MDA-MB-231 has been used to study:
  • the changes in the morphological pattern and gene expression of cells when grown in the presence of sera positive for anti-carbonic anhydrase I (CA I) autoantibodies[1]
  • the effects of normothermic conditioned microwave irradiation on cultured cells using an irradiation system[2]
  • the transendothelial migration rates of the cells under different shear stress levels[3]

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18

배양 배지

L15+ 2mM Glutamine + 15% Foetal Bovine SerumFBS / FCS.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm2 using 0.05% Trypsin or Trypsin/EDTA; 37°C. CO2 is not required.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

면책조항

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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시험 성적서(COA)

Lot/Batch Number

죄송합니다. 지금은 이 제품에 대한 COA이(가) 온라인에서 제공되지 않습니다.

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문서 라이브러리 방문

문서

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

질문

1–9 / 9 질문  

  1. Gent.mi, il codice prodotto si riferisce alla linea cellulare o al DNA estratto da essa? Grazie

    1 답변

    1. Product 92020424-1VL is for the cells themselves, MDA-MB-231 Cell Line human. Product 92020424-DNA-5UG is for the corresponding DNA.

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  2. Doubts about complete growth media that I have to use with MDA-MB-231. Is it L15 + 2 mM Glutamine+10% FBS or Can I use DMEM, 10% FBS, PenStrep? Protocol that I have to follow after thawing cells (volume, rpm, V to resuspend, FlaskT25 or T75 and ratio)

    1 답변

    1. It is best to adhere to the guidelines recommended by ECACC, at least for bringing cells out of the freeze. Some end users have successfully transitioned cells to other media, but this should always be a gradual transition over several sub-cultures to ensure robust cell growth and morphology.

      For this cell line see this list of products that will meet the supplier's requirements:
      L-15 Leibovitz Medium – L1518 Sterile Liquid or L4386 Powdered Medium (with L-Glutamine)
      https://www.sigmaaldrich.com/product/sigma/l1518
      https://www.sigmaaldrich.com/product/sigma/l4386

      FBS – F2442
      https://www.sigmaaldrich.com/product/sigma/f2442

      Trypsin – T4424
      https://www.sigmaaldrich.com/product/sigma/t4424

      Trypsin EDTA – T4049
      https://www.sigmaaldrich.com/product/sigma/t4049

      The traditionalists advise avoiding using antibiotics, however, if impeccable aseptic technique is a challenge and busy labs and incubators are common, antibiotics are acceptable. Should the added assurance of Pen/Strep be preferred, see product P0781:
      https://www.sigmaaldrich.com/product/sigma/p0781

      The T25 flasks would be best for starting up cultures. After the culture is established, end users may prefer the T75 flasks, provided there is adequate cell density.

      See the link below to the supplier product information for additional information and details:
      https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=92020424&collection=ecacc_gc

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  3. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 답변

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

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  4. How many Cell Line cells are in the vial?

    1 답변

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

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  5. What medium should I use when I thaw the Cell Line cells?

    1 답변

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

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  6. What passage are my Cell Line cells?

    1 답변

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

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  7. Are the Cell Line cells tested for viral pathogens?

    1 답변

    1. HPA Cultures does not perform any viral testing on the cell lines.

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  8. Are my Cell Line cells mycoplasma tested?

    1 답변

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

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  9. How do I handle Cell Line frozen cells upon arrival?

    1 답변

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

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