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L5520

L-15 Medium (Leibovitz)

Without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Leibovitz L15

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217482
Pricing and availability is not currently available.
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Quality Level

500

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L

shipped in

ambient

storage temp.

2-8°C

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This Item
L1518L4386M9309
Sigma-Aldrich

Sigma-Aldrich

L5520

L-15 Medium (Leibovitz)

Sigma-Aldrich

Sigma-Aldrich

L1518

L-15 Medium (Leibovitz)

Sigma-Aldrich

Sigma-Aldrich

L4386

L-15 Medium (Leibovitz)

McCoy’s 5A Medium Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M9309

McCoy′s 5A Medium

form

liquid

form

liquid

form

powder

form

liquid

components

phenol red: 0.011 g/L
sodium pyruvate: 0.55 g/L

components

phenol red: 0.011 g/L
L-glutamine: 0.3 g/L
sodium pyruvate: 0.55 g/L

components

sodium pyruvate: 0.55 g/L
phenol red: 0.011 g/L
L-glutamine: 0.3 g/L

components

NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

L-15 Medium (Leibovitz) was initially prepared to be used in carbon dioxide (CO2) free systems, which involves sodium bicarbonate. The complement of free base amino acids, salts, and galactose buffers it. This aids the maintenance of physiological pH control.

Application

L-15 Medium (Leibovitz) has been used:  
  • in protein incorporation for incubation of high blastula embryos and to avoid the abnormal development of embryos[1]
  • as a supplement in sterile cell media for the dissection and isolation of kidney cells[2]
  • as a component of culture buffer for the tissue culture of Newt lung cells[3]

Preparation Note

Supplement with 0.3 g/L L-glutamine.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000426113
0000426113
0000364988
0000364988
0000353578

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Francisco J Monje et al.
Annals of medicine, 45(4), 301-307 (2013-05-02)
Studies of synaptic plasticity using the marine mollusk Aplysia californica as model system have been successfully used to identify proteins involved in learning and memory. The importance of molecular elements regulated by the learning- related neurotransmitter serotonin in Aplysia can
Applications of cell lines as bioreactors and in vitro models
Singh J and Goswami A
International journal of applied & basic medical research null
Olga Krylyshkina et al.
The Journal of cell biology, 156(2), 349-359 (2002-01-25)
Recent studies have shown that the targeting of substrate adhesions by microtubules promotes adhesion site disassembly (Kaverina, I., O. Krylyshkina, and J.V. Small. 1999. J. Cell Biol. 146:1033-1043). It was accordingly suggested that microtubules serve to convey a signal to
B Houchmandzadeh et al.
The Journal of cell biology, 139(1), 1-12 (1997-10-06)
The structure of mitotic chromosomes in cultured newt lung cells was investigated by a quantitative study of their deformability, using micropipettes. Metaphase chromosomes are highly extensible objects that return to their native shape after being stretched up to 10 times
Ya Gao et al.
Fish physiology and biochemistry, 45(3), 1129-1139 (2019-03-20)
A continuous fibroblast-like cell line, TMF (turbot muscle fibroblasts), was established from juvenile turbot Scophthalmus maximus muscle with the method of trypsin digestion. It has been subcultured more than 60 passages for over 150 days. The TMF cells were cultured in
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Global Trade Item Number

SKUGTIN
L5520-500ML04061833958223
L5520-100ML04061833958209
L5520-24X500ML04061833958216
L5520-6X500ML04061833958230

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