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Merck
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문서

M2279

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

동의어(들):

EMEM, MEM

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

product name

Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

무균

sterile-filtered

형태

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

성분

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

배송 상태

ambient

저장 온도

2-8°C

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일반 설명

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. It includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for the cultivation of a wide variety of cells grown in monolayers and attached cell lines, such as fibroblasts, in the presence of fetal bovine serum (FBS), calf, or horse sera.

애플리케이션

Minimum Essential Medium Eagle has been used to culture the:
  • explants of aortic valve leaflets
  • human liver cancer cell line (HepG2) cells
  • human fetal brain progenitor-derived astrocyte cells (PDA)
  • breast cancer cell line (MCF-7)
  • human glioblastoma cell line (T98G)

제조 메모

Supplement with 0.292 g/L L-glutamine.

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보충제

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추천

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Winston X Yan et al.
Nature communications, 8, 15058-15058 (2017-05-13)
Precisely measuring the location and frequency of DNA double-strand breaks (DSBs) along the genome is instrumental to understanding genomic fragility, but current methods are limited in versatility, sensitivity or practicality. Here we present Breaks Labeling In Situ and Sequencing (BLISS)
Kaisa-Emilia Makkonen et al.
Journal of virology, 87(20), 11148-11159 (2013-08-09)
Baculoviruses are insect-specific viruses commonly found in nature. They are not able to replicate in mammalian cells but can transduce them when equipped with an appropriate mammalian cell active expression cassette. Although the viruses have been studied in several types
Emily R Trzeciak et al.
Cells, 11(6) (2022-03-26)
The cellular composition of the tumor microenvironment, including tumor, immune, stromal, and endothelial cells, significantly influences responses to cancer therapies. In this study, we analyzed the impact of oxidative stress, induced by cold atmospheric plasma (CAP), on tumor cells, T
Luciano A Masullo et al.
Nature communications, 9(1), 3281-3281 (2018-08-18)
The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging
Natalia Papargyri et al.
Molecular therapy. Nucleic acids, 19, 706-717 (2020-01-18)
The identification of molecules that can modulate RNA or protein function and the subsequent chemical and structural optimization to refine such molecules into drugs is a key activity in drug discovery. Here, we explored the extent to which chemical and
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