MIA-Pa-Ca-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85062806, human pancreas, Epithelial

• 동의어(들): MIA PaCa-2 Cells, MIA-PaCa2 Cells, MIAPACA-2 Cells, PaCa2 Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human pancreas
• 포장: tube of 5 μg 85062806-DNA-5UG; pkg of vial of cells 85062806-1VL
• 성장 모드: Adherent
• 핵형: Modal no. 61; hypotriploid
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian pancreatic carcinoma

세포주 설명

An established cell line from an undifferentiated human pancreatic carcinoma. The tumour was taken from a 65 year old Caucasian male. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy, have a tendency to grow on top of other cells, eventually growing free in suspension. Sensitive to L-Asparaginase.

애플리케이션

MIA-Pa-Ca-2 has been used for the assessment of thyroid receptor transcriptional activity and for α-enolase immunostaining.[1][2]

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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