HPLC Analysis of Protein Standards on Zenix® SEC-300 in the Presence of a Denaturant
材料
Standard
製品番号
詳細
価格
分析カラム
製品番号
詳細
価格
Zenix® SEC-300 Gel Filtration Column, 3 μm
L × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steelCONDITIONS
column
Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)
mobile phase
[A] 0.1 M ammonium bicarbonate (pH unadjusted); [B] 0.1 M ammonium bicarbonate (pH unadjusted) with 6 M guandine hydrochloride (pH unadjusted)
flow rate
0.3 mL/min
pressure
1300 psi (90 bar)
column temp.
25 °C
detector
UV, 280 nm
injection
2.5 μL
sample
Thyroglogulin, 1.5 g/L; IgG, 1 g/L; Albumin, 2.5 g/L; Myoglobin, 1 g/L; Uracil, 0.05 g/L; in mobile phase
詳細
アナリシスノート
This application compares selectivity of proteins when chromotographed by size exclusion in the presence and absence of a denaturant (guandine-HCl).
法的情報
Zenix is a registered trademark of Sepax Technologies