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  • A comparative study on the aggregating effects of guanidine thiocyanate, guanidine hydrochloride and urea on lysozyme aggregation.

A comparative study on the aggregating effects of guanidine thiocyanate, guanidine hydrochloride and urea on lysozyme aggregation.

Biochemical and biophysical research communications (2014-07-09)
Saeed Emadi, Maliheh Behzadi
要旨

Protein aggregation and its subsequent deposition in different tissues culminate in a diverse range of diseases collectively known as amyloidoses. Aggregation of hen or human lysozyme depends on certain conditions, namely acidic pH or the presence of additives. In the present study, the effects on the aggregation of hen egg-white lysozyme via incubation in concentrated solutions of three different chaotropic agents namely guanidine thiocyanate, guanidine hydrochloride and urea were investigated. Here we used three different methods for the detection of the aggregates, thioflavin T fluorescence, circular dichroism spectroscopy and atomic force microscopy. Our results showed that upon incubation with different concentrations (0.5, 1.0, 2.0, 3.0, 4.0, 5.0M) of the chemical denaturants, lysozyme was aggregated at low concentrations of guanidine thiocyanate (1.0 and 2.0M) and at high concentrations of guanidine hydrochloride (4 and 5M), although no fibril formation was detected. In the case of urea, no aggregation was observed at any concentration.

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製品内容

Sigma-Aldrich
グアニジン 塩酸塩, for molecular biology, ≥99%
Sigma-Aldrich
グアニジン 塩酸塩, ≥98%
Sigma-Aldrich
尿素, powder, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
グアニジン 塩酸塩, organic base and chaeotropic agent, ≥99% (titration)
Sigma-Aldrich
尿素 溶液, BioUltra, ~8 M in H2O
Supelco
Urea, 8 M (after reconstitution with 16 mL high purity water)
Sigma-Aldrich
チオフラビンT, used as stain for amyloid
SAFC
グアニジン 塩酸塩
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尿素, BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)
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尿素, BioUltra, for molecular biology, 99% (T)
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グアニジン 塩酸塩 溶液, Colorless liquid, 7.8 - 8.3 M, pH- 4.5 - 5.5
Sigma-Aldrich
尿素, suitable for electrophoresis
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尿素, ACS reagent, 99.0-100.5%
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グアニジン 塩酸塩 溶液, BioUltra, ~8 M in H2O
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グアニジン 塩酸塩, ≥99.0% (AT)
USP
尿素, United States Pharmacopeia (USP) Reference Standard
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グアニジン 塩酸塩, BioUltra, for molecular biology, ≥99.5% (AT)
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グアニジンチオシアナート, BioReagent, for molecular biology, ≥99%
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尿素, meets USP testing specifications
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グアニジン 塩酸塩, anhydrous, free-flowing, Redi-Dri, ≥99%
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尿素 溶液, 40 % (w/v) in H2O
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グアニジンチオシアナート, for molecular biology, ≥99%
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尿素, ReagentPlus®, ≥99.5%, pellets
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グアニジンチオシアナート, BioUltra, for molecular biology, ≥99.0% (AT)
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尿素, ≥99.0%
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尿素, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)
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Urea-12C, 99.9 atom % 12C
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尿素 溶液, suitable for microbiology, 40% in H2O
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尿素, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%
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グアニジンチオシアナート, ≥97% (titration)