SRP6082

TRXB from Escherichia coli

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 別名: TRXR, Thioredoxin reductase
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: Escherichia coli
• リコンビナント: expressed in E. coli
• アッセイ: ≥90% (SDS-PAGE)
• フォーム: liquid
• 分子量: 34.6 kDa
• 包装: pkg of 100 μg
• NCBIアクセッション番号: P0A9P4
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: Escherichia coli ... TRXB(949054)

詳細

詳細

Thioredoxin reductase (TrxR) is encoded by TRXB gene. TrxR is one of the member of flavoprotein family of pyridine nucleotide-disulphide oxidoreductases, which also includes lipoamide dehydrogenase, glutathione reductase and mercuric ion reductase. Escherichia coli TrxR is a 70-kDa homodimeric flavoprotein, each monomer of this protein contains a FAD prosthetic group, an NADPH domain and an active site with redox-active disulphide. The catalytic site (-Cys-Ala-Thr-Cys-) in Escherichia coli TrxB is localized on the NADPH domain, whereas in humans TrxB catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) is part of the FAD domain.[1][2]

生物化学的／生理学的作用

Thioredoxin reductase (TRXB) catalyzes the reduction of oxidized thioredoxin using nicotinamide adenine dinucleotide phosphate (NADPH).[1][3] In thioredoxin pathway, this reduced thioredoxin reduce disulfide bonds in proteins localized in the cytoplasm and is implicated in the recycling of an essential enzyme ribonucleotide reductase. TrxR is essentially involved in protection against oxidation stress, cell growth and transformation, and the recycling of ascorbate from its oxidized form.[1]

物理的形状

1 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 1mM DTT.

調製ノート

バイアルは、開封前に遠心してください。

その他情報

MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H319
• 注意書き: P264 - P280 - P305 + P351 + P338 - P337 + P313
• 危険有害性の分類: Eye Irrit. 2
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable