SRP0255

eIF4E Active human

N-terminal GST-tag, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• 別名: CBP
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥70% (SDS-PAGE)
• フォーム: aqueous solution
• 分子量: 51 kDa
• 包装: pkg of 100 μg
• 濃度: >0.02 mg/mL
• テクニック: cell based assay: suitable
• 適合性: suitable for molecular biology
• NCBIアクセッション番号: NM_001968
• UniProtアクセッション番号: P06730
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... EIF4E(1977)

詳細

詳細

EIF4E (eukaryotic translation initiation factor 4E) is a m7GpppN (N is any nucleotide) cap-dependent RNA-binding protein.[1] It is one of the three subunits of the translation initiation factor complex eIF4F, and is the least common of all initiation factors.[2][3]

アプリケーション

Suitable substrate for MNK1 kinase.

生物化学的／生理学的作用

EIF4E (eukaryotic translation initiation factor 4E) is a part of eIF4F complex, which is responsible for the recruitment of ribosomes to the capped end of mRNAs during mRNA translation. It acts as the rate-limiting element in cap-dependent mRNA translation in eukaryotic cells. It promotes the translation of pro-oncogenic mRNAs, including vascular endothelial growth factor (VEGF), cyclin D3, and Mcl-1 (induced myeloid leukemia cell differentiation). Up-regulation of this protein is linked with various cancers, such as prostate cancer, breast cancer, and lymphoma. Studies show that this protein is overexpressed in melanoma cell lines and is linked with resistance to vemurafenib. Thus, it might have potential as a therapeutic target to suppress melanoma cell proliferation and abrogating vemurafenib resistance.[1][4]

物理的形状

Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注された希釈前の酵素を-70°Cで保存してください。注記:酵素は凍結解凍の繰り返しに非常に弱いので、注意してください。

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable