RDP200

GenElute^™ FFPE RNA/DNA Purification Plus Kit

• UNSPSCコード: 41105600
• NACRES: NA.52

特性

• 環境により配慮した代替製品の特徴: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 環境により配慮した代替製品カテゴリ: Aligned,
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

Sigma′s GenElute^™ FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Sigma′s GenElute^™ FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis.

• Fast and easy processing using rapid spin-column format
• High yields and Quality of Nucleic Acids
• Separate fractionation of RNA and DNA
• Isolate total RNA, from large rRNA down to microRNA (miRNA)
• No phenol or chloroform extractions

アプリケーション

RNA

• Bioanalyzer
• Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
• RT-PCR for both large mRNA and small RNA including miRNA
• Northern blotting
• RNase protection
• Primer extension
• Expression array assays
• Next Generation Sequencing
• microRNA Cloning
• miRNA array
• RNA-Seq
• Nanostring
• Fluidigm
• Droplet/Digital PCR

Genomic DNA

• PCR and Quantitative PCR
• Sequencing
• DNA Methylation Studies
• Southern blotting
• Microarrays
• SNP analysis

特徴および利点

• Complete column purification - Separate fractionation of RNA and DNA
• Reduce variability - RNA and DNA are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variability
• Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA
• Isolate high quality RNA and DNA - The purified RNA and DNA are of the highest quality and can be used in a number of downstream applications

単位の定義

Preps

法的情報

GenElute is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• ピクトグラム: GHS08,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H302 + H332,H314,H334,H412
• 注意書き: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1C
• 補足的ハザード: EUH032
• 保管分類コード: 8A - Combustible corrosive hazardous materials