R5780

Anti-Rab4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-HRES-1/RAB4, Anti-RAB4A, Anti-RAS-associated protein RAB4
• MDL番号: MFCD01634072
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~24 kDa
• 化学種の反応性: human, rat, mouse
• 濃度: ~1.0 mg/mL
• テクニック: indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 and rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.1% and 0.4% saponin, respectively.; western blot: 2-5 μg/mL using whole extract of human HEK-293T cells
• UniProtアクセッション番号: P20338
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... RAB4A(5867) ; mouse ... Rab4a(19341) ; rat ... Rab4a(25532)

詳細

詳細

Rab4 is a GTPase , that is located in early and recycling endosomes. It belongs to Rab family and is located on human chromosome 1q42. In rat testis, Rab4 is found in sertoli and germ cells.

There are two highly homologous RAB4 isoforms, RAB4A and RAB4B, encoded by two separate genes (RAB4A and RAB4B). RAB4A and RAB4B are localized in the same cellular compartments and are believed to have similar or identical functions in recycling.

アプリケーション

The antibody can be used in several immunochemical techniques including immunoblotting (24 kDa) and immunofluorescence.

生物化学的／生理学的作用

Rab4 regulates endocytic recycling in a cell cycle dependent fashion. Rab4 is associated with early endosomes and recycling endosomes and regulates the recycling of membranes and proteins from these compartments back to the plasma membrane. Rab4 interacts with several Rab4 effector proteins like Rabip4, that regulates integrin trafficking and is involved in the migration of NIH 3T3 fibroblasts.

Rab4a controls integrin trafficking throughout cell adhesion. It helps in the quick reprocess of receptors from early endosomes to the cell surface. It also controls the secretion of procathepsin L in human melanoma cells.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)