R0280

Anti-RanGAP1 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution, ~2 mg/mL

• 別名: Anti-Ran-GTPase-Activating Protein 1
• MDL番号: MFCD09265372
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~65 kDa
• 化学種の反応性: human
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~2 mg/mL
• テクニック: indirect immunofluorescence: 10-20 μg/mL using HeLa cells; western blot: 1-2 μg/mL using HEK-293T cells transfected with human RanGAP1
• UniProtアクセッション番号: P46060
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... RANGAP1(5905) ; mouse ... Rangap1(19387)

詳細

詳細

Ran GTPase−activating protein 1 (RanGAP1), a guanosine-5′-triphosphatase (GTPase)-activating protein for Ran exists in two different types, where one is cytoplasmic and other is concerted at the cytoplasmic fibers of nuclear pore complexes (NPCs). Mammalian RanGAP1 has a C-terminal extension. Ran GTPase gene is located on human chromosome 22q13.2.

特異性

Anti-RanGAP1 (N-terminal) recognizes human RanGAP1.

免疫原

synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of human RanGAP1, conjugated to KLH. This sequence is identical in mouse RanGAP1.

アプリケーション

Anti-RanGAP1 (N-terminal) antibody produced in rabbit may be used immunoblotting and immunofluorescence.

生物化学的／生理学的作用

Ran GTPase−activating protein 1 (RanGAP1) regulates Ran activity. RanGAP1 can stimulate the GTPase activity of Ran. Ran is also regulated by a chromatin-bound nucleotide exchange factor, regulator of chromosome condensation 1 (RCC1) that keeps Ran in the active guanosine-5′-triphosphate (GTP)-bound state. RanGAP1 has the ability to conjugate to the small ubiquitin-related modifier protein small ubiquitin-related modifier 1 (SUMO-1). RanGAP1 may participate in nuclear protein import.

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)