M6667

単球走化性タンパク質-1 ヒト

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 別名: MCP-1
• MDL番号: MFCD00131621
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in E. coli
• アッセイ: ≥97% (SDS-PAGE and N-terminal analysis)
• フォーム: lyophilized powder
• 有効性: 5-60 ng/mL EC₅₀
• 分子量: 8.7 kDa
• 包装: pkg of 10 μg
• テクニック: cell culture | mammalian: suitable
• 不純物: endotoxin, tested
• UniProtアクセッション番号: P13500
• 保管温度: −20°C
• 遺伝子情報: human ... CCL2(6347)

詳細

詳細

Monocyte chemoattractant protein-1 (MCP-1) or monocyte chemotactic and activating factor (MCAF) is part of the C-C chemokine family. [1] The gene encoding this protein is localized on human chromosome 17q12.[2]

アプリケーション

Monocyte chemotactic protein-1 (MCP-1) has been used to study its effects on the motility of lymphoblastoid cell line NC–NC cells.

生物化学的／生理学的作用

The biological activity is measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A. The ED<SUB>50</SUB> for this effect is typically 5-30 ng/mL.

Measured by its ability to chemoattract 2-day cultured human monocytes, Matsushima, K., et al. (1989) J. Exp. Med. 169:1485, the ED<SUB>50</SUB> for this effect is typically 5-20 ng/mL.

Monocyte chemoattractant protein-1 (MCP-1) triggers the activation of monocytes by triggering degranulation as well as respiratory burst activity in monocytes. It facilitates monocytes in inhibiting or enhancing the growth of specific brain tumor cell lines. MCP-1 chemoattracts monocytes, macrophages, T cells and natural killer cells. The protein is overexpressed in atherosclerosis, idiopathic pulmonary fibrosis, rheumatoid arthritis and delayed hypersensitivity reactions. It is also implicated in Kawasaki disease.[1][3]

物理的形状

凍結乾燥品 (PBS溶液, 0.5mg BSA含有, 0.2μmフィルタ-滅菌済み)。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)