L1378

L-乳酸デヒドロゲナーゼ ウシ筋肉由来

Type X, ammonium sulfate suspension, ≥600 units/mg protein

• 別名: (S)-乳酸: NAD⁺ オキシドレダクターゼ, L-LDH, LD, LDH
• CAS番号: 9001-60-9
• Enzyme Commission number: 1.1.1.27 (BRENDA, IUBMB)
• EC Number: 232-617-8
• MDL番号: MFCD00131466
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: bovine muscle
• タイプ: Type X
• アッセイ: 3.0—25.0 mg protein/mL (biuret)
• フォーム: ammonium sulfate suspension
• 比活性: ≥600 units/mg protein
• メーカー／製品名: Sigma-Aldrich
• テクニック: activity assay: suitable
• 色: white
• pH: 7.5
• UniProtアクセッション番号: P19858; Q5E9B1
• アプリケーション: life science and biopharma
• その他の活性: pyruvate kinase ≤0.01%
• 保管温度: 2-8°C
• 遺伝子情報: cow ... LDHA(281274) , LDHB(281275)

詳細

詳細

Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.[1]

アプリケーション

L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.[2]

生物化学的／生理学的作用

また、他のL-2-hydroxymonocarboxylic acidの酸化を触媒します。

Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.[1]

単位の定義

1ユニットは、pH 7.5、37°C、1分間で、1.0 μmolのピルビン酸をL-乳酸へと還元する酵素量です。

物理的形状

pH 6.0の2.4 M (NH_}4)₂SO₄溶液への結晶性懸濁液

アナリシスノート

タンパク量はビウレット法で測定

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)