SAE0049

Lactic Dehydrogenase, recombinant 大腸菌由来

from human, recombinant, expressed in E. coli, aqueous solution

• 別名: (S)-ラクテート：NAD⁺オキシドレダクターゼ, L-ラクテートデヒドロゲナーゼ, 乳酸
• CAS番号: 9001-60-9
• Enzyme Commission number: 1.1.1.27 (BRENDA, IUBMB)
• MDL番号: MFCD00131466
• UNSPSCコード: 12352200
• NACRES: NA.54

特性

• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in E. coli
• フォーム: aqueous solution
• 保管条件: (Keep container tightly closed in a dry and well-ventilated place)
• 色: colorless
• UniProtアクセッション番号: K1T0A2
• 輸送温度: dry ice
• 保管温度: −20°C
• 遺伝子情報: human ... LDHA(3939)

詳細

詳細

Research area: Cell Signaling
The gene LDHA (L-lactate dehydrogenase A chain) is mapped to human chromosome 11p15.[1] It is a subunit of lactate dehydrogenase.[2]In particular, lactic dehydrogenase A (LDHA) is mainly found in skeletal muscle, and for that reason is known as the M subunit. This recombinant form of LDHA has a C-terminal histidine-tag.

アプリケーション

L-Lactate Dehydrogenase (LDHA) has been used in in vitro phosphoglycerate mutase 1 (PGAM1) inhibitors screening assay.[3] It has also been used in a colorimetric assay for determining lactate concentration in conditioned media.[4]

生物化学的／生理学的作用

L-乳酸デヒドロゲナーゼは、L-乳酸をL-ピルビン酸に変換する反応を触媒しながら、NAD⁺を還元してNADHおよびH⁺を生成します。

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.[5]  L-lactate dehydrogenase A chain (LDHA), an enzyme involved in pyruvate metabolism,[6] LDH is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers.[6][7] LDHA regulates the microenvironment of developing tumors by the hypoxia-inducible factor (HIF)-signaling pathway.[7] LDHA aids in the NAD⁺ regeneration during the β-oxidation of fatty acid.[8] LDHA (L-lactate dehydrogenase A chain) is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. In cancer cells, HIF-1a (hypoxia-inducible factor) induces the expression of LDHA, which helps in maintaining glycolysis in cells.[2]

単位の定義

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

物理的形状

Buffered aqueous solution with Hepes (pH 7.5), NaCl and glycerol.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable