Insulin has low solubility at neutral pH. It can be solubilized at 1-10 mg/mL in dilute acetic (1%) or hydrochloric acid, pH 2-3. Insulin can also be solubilized in 125 mM NaHCO3.15 However, alkaline stock solutions are not recommended since high pH increases the rate of deamidation and aggregation.
I1882
インスリン ウシ膵臓由来
γ-irradiated, BioXtra, suitable for cell culture, potency: ≥20 units/mg (USP units), lyophilized powder
別名:
Bovine insulin, Cell culture grade insulin
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100 MG
￥42,100
About This Item
実験式（ヒル表記法）:
C254H377N65O75S6
CAS番号:
分子量:
5733.49
EC Number:
MDL番号:
UNSPSCコード:
12352209
NACRES:
NA.77
おすすめの製品
由来生物
bovine
品質水準
無菌性
γ-irradiated
製品種目
BioXtra
フォーム
lyophilized powder
有効性
≥20 units/mg (USP units)
テクニック
cell culture | mammalian: suitable
溶解性
acetic acid: 2 mg/mL (pH: 2-3)
hydrochloric acid: 2 mg/mL (pH: 2-3)
UniProtアクセッション番号
輸送温度
ambient
保管温度
−20°C
InChI Key
IXIBAKNTJSCKJM-BUBXBXGNSA-N
遺伝子情報
bovine ... INS(280829)
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関連するカテゴリー
アプリケーション
インスリンは多くの哺乳類細胞培養系で成長因子として使用されます。
生物化学的／生理学的作用
膵島のβ細胞によって産生される、2本のポリペプチド鎖からなるホルモンです。分子量は、約5800 Daです。αおよびβ鎖が、2鎖間のジスルフィド結合によって結合しています。α鎖は、鎖内のジスルフィド結合を持っています。インスリンは、グルコース、アミノ酸、および脂肪酸の、細胞への取り込み、利用、および貯蔵を調節し、グリコーゲン、タンパク質、および脂肪の分解を阻害します。
その他情報
InChI: 1S/C254H377N65O75S6/c1-29-131(24)205(312-192(334)103-256)250(389)317-204(130(22)23)246(385)287-158(74-81-199(344)345)216(355)281-155(70-77-188(260)330)220(359)307-183-116-399-400-117-184-242(381)305-177(110-321)238(377)293-161(87-122(6)7)223(362)294-167(94-139-52-60-145(324)61-53-139)226(365)282-153(68-75-186(258)328)217(356)289-160(86-121(4)5)221(360)284-157(73-80-198(342)343)219(358)301-173(100-189(261)331)233(372)297-169(96-141-56-64-147(326)65-57-141)229(368)308-182(241(380)303-175(253(393)394)102-191(263)333)115-398-396-113-180(212(351)270-106-193(335)277-152(71-78-196(338)339)215(354)280-150(50-41-83-268-254(264)265)210(349)269-107-194(336)278-165(92-137-45-35-31-36-46-137)225(364)296-166(93-138-47-37-32-38-48-138)228(367)298-170(97-142-58-66-148(327)67-59-142)236(375)318-206(135(28)323)251(390)319-84-42-51-185(319)244(383)285-151(49-39-40-82-255)213(352)276-134(27)252(391)392)310-248(387)202(128(18)19)315-234(373)163(89-124(10)11)291-227(366)168(95-140-54-62-146(325)63-55-140)295-222(361)159(85-120(2)3)288-207(346)132(25)274-214(353)156(72-79-197(340)341)286-245(384)201(127(16)17)314-235(374)164(90-125(12)13)292-231(370)172(99-144-105-267-119-273-144)300-237(376)176(109-320)279-195(337)108-271-211(350)179(112-395-397-114-181(309-243(183)382)240(379)275-133(26)208(347)304-178(111-322)239(378)316-203(129(20)21)249(388)311-184)306-224(363)162(88-123(8)9)290-230(369)171(98-143-104-266-118-272-143)299-218(357)154(69-76-187(259)329)283-232(371)174(101-190(262)332)302-247(386)200(126(14)15)313-209(348)149(257)91-136-43-33-30-34-44-136/h30-38,43-48,52-67,104-105,118-135,149-185,200-206,320-327H,29,39-42,49-51,68-103,106-117,255-257H2,1-28H3,(H2,258,328)(H2,259,329)(H2,260,330)(H2,261,331)(H2,262,332)(H2,263,333)(H,266,272)(H,267,273)(H,269,349)(H,270,351)(H,271,350)(H,274,353)(H,275,379)(H,276,352)(H,277,335)(H,278,336)(H,279,337)(H,280,354)(H,281,355)(H,282,365)(H,283,371)(H,284,360)(H,285,383)(H,286,384)(H,287,385)(H,288,346)(H,289,356)(H,290,369)(H,291,366)(H,292,370)(H,293,377)(H,294,362)(H,295,361)(H,296,364)(H,297,372)(H,298,367)(H,299,357)(H,300,376)(H,301,358)(H,302,386)(H,303,380)(H,304,347)(H,305,381)(H,306,363)(H,307,359)(H,308,368)(H,309,382)(H,310,387)(H,311,388)(H,312,334)(H,313,348)(H,314,374)(H,315,373)(H,316,378)(H,317,389)(H,318,375)(H,338,339)(H,340,341)(H,342,343)(H,344,345)(H,391,392)(H,393,394)(H4,264,265,268)/t131-,132-,133-,134-,135+,149-,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,200-,201-,202-,203-,204-,205-,206-/m0/s1
物理的形状
粉末-0°C保存。溶液は 2～-8°Cで 1年間安定です。
再構成
10 mg/mLのストック溶液を調製するには、氷酢酸(約0.1 mL)添加により酸性化したH2O(pH≦2)10 mLを加えます。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
I1882-PH:
I1882-100MG-IR:
I1882-VAR:
I1882PROC:
I1882-BULK:
I1882-100MG:
I1882-100MG-PW:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
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how to soluble i1882? What percent dilution should I use to gial acetic acid?
1 回答-
役に立ちましたか？
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What can be used to make a solution of insulin?
1 回答-
Insulin is soluble in dilute acids and alkalis. The solubility of insulin is tested at 2 mg/mL in water (acidified to pH 2 with hydrochloric acid). One can also use 1 mL of water acidified to pH 2 with 10 μL of glacial acetic acid to dissolve 2 mg of insulin. A 10 mg/mL solution of bovine insulin in 25 mM HEPES at pH 8.2 is offered as Product No. I0516.
役に立ちましたか？
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How many units of insulin are present in one vial of Product I1882, Insulin from bovine pancreas?
1 回答-
Product No. I1882 has a potency specification of not less than 2520 USP units per vial. For a vial that contains (nominally) 100 mg, that works out to 25.2 USP units/mg. The potency in terms of units per vial can be found the on lot-specific Certificate of Analysis.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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How can I store the insulin solution? How long?
1 回答-
The USP recommends that solutions of insulin be stored at 2-8°C. It is not recommended to freeze solutions of insulin, as it can aggregate. Sterile solutions should be stable for at least 1 year at 2-8°C.
役に立ちましたか？
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What is the amino acid sequence for Product I1882, Insulin from bovine pancreas?
1 回答-
The sequence and structure of insulin can be found on the product information sheet (under Documents, above).
役に立ちましたか？
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What is the comparison between International Units (IU) and USP units for insulin?
1 回答-
The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.
役に立ちましたか？
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