H1136 is not supplied in solution form. The product is lyophilized directly into the ampule and its appearance is a clear, thin film.
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由来生物
Streptomyces sp. (S. hyalurolyticus)
品質水準
結合体
conjugate (Glucosaminoglycan)
フォーム
film (Clear)
包装
ampule of 300 × units
保管温度
−20°C
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関連するカテゴリー
詳細
アプリケーション
生物化学的／生理学的作用
ヒアルロニダーゼはエンドグリコシダーゼであり、β-グルクロニダーゼやβ-N-アセチルグルコサミニダーゼなどのエキソグリコシダーゼとともに機能します。ヒアルロニダーゼは、ヒアルロン酸の代謝回転を制御します。[1]
ヒアルロン酸リアーゼは、β-D-GlcNAc-（1→4）-β-D-GlcA結合でヒアルロン酸を切断し、3-（4-デオキシ-β-D-グルコ-4-エヌロノシル）-N-アセチル-D-グルコサミン四糖および六糖を生成します。他のヒアルロニダーゼとは異なり、この酵素はヒアルロン酸に特異的で、コンドロイチンおよびコンドロイチン硫酸に対しては不活性です。[4]
単位の定義
1単位は、37ºC、pH 5.35で1分間に0.75 μgの多糖ヒアルロン酸を分解させます（45分後のBSAとの複合体形成時に比濁吸光法（λ：600 nm）によって測定）。
その他情報
より詳細な情報につきましては、www.sigma-aldrich.com/enzymeexplorerのenzymes for complex carbohydrate analysisをご覧ください。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
H1136-VAR:
H1136-1AMP:
H1136-1AMP-PW:
H1136-BULK:
H1136-5X1AMP:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
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資料
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
プロトコル
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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What is the volume in each vial/ampoule?
1 回答-
役に立ちましたか？
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You mention the following buffer composition for reconstitution: "...20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0". If I want to refreeze aliquots, would you suggest the same buffer? Isn't sodium phosphate harmful here?
1 回答-
Long-term solution stability studies have not been performed on the microbial source hyaluronidase. However, the bovine source products are stable for up to 1 month at 4 deg. C (500 mM acetate buffer pH 6.0) and several months at - 20 deg. C. Aliquot solutions to store frozen, avoid repeated freeze/thaw cycles. The recommended reconstitution buffer should include 0.01% BSA which acts as a stabilizer, there is no indication that the recommended sodium phosphate/sodium chloride buffer would be inappropriate for freezing.
役に立ちましたか？
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What is the molecular weight of Product H1136, Hyaluronidase?
1 回答-
The molecular weight for hyaluronidase from Streptomyces hyalurolyticus has been reported to be 91,000 DALTONS as determined by gel filtration.
役に立ちましたか？
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How should Product H1136, Hyaluronidase, be reconstituted?
1 回答-
Prior to determining the activity of this enzyme, we reconstitute it in 20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0.
役に立ちましたか？
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How is the activity of Product H1136, Hyaluronidase,determined?
1 回答-
Sigma assays the enzyme against a USP hyaluronidase reference standard as described in USP XXII-NF XVII, 644 (1990).
役に立ちましたか？
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What amount of product is present in the vial of Product H1136, Hyaluronidase?
1 回答-
The dry weight of the product is not determined. The vial should contain a minimum of 300 units of enzyme.
役に立ちましたか？
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I can't see any material in the container. Is material present in the container?
1 回答-
The product is lyophilized directly into the ampule and its appearance is a clear, thin film.
役に立ちましたか？
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Are any other components present in Product H1136, Hyaluronidase, besides the enzyme?
1 回答-
No protein or BSA has been added as a stabilizer. The enzyme was lyophilized from 0.5 mM Tris buffer, pH 7.0.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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