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GE28-9670-56

Protein A Mag Sepharose™ Xtra

Cytiva 28-9670-56, pack of 2 × 1 mL

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About This Item

UNSPSCコード:
41106500
NACRES:
NA.56
現在、価格および在庫状況を閲覧できません。

ligand

native protein A

包装

pack of 2 × 1 mL

メーカー／製品名

Cytiva 28-9670-56

保管条件

(20% Ethanol, 10% medium slurry)

パラメーター

Room temperature temp. range

Matrix

paramagnetic, spherical, highly cross-linked agarose particles

キャパシティ

27 mg binding capacity(human IgG/ml gel)

保管温度

2-8°C

関連するカテゴリー

詳細

protein A Mag Sepharose™ are magnetic beads designed to simplify small-scale purification and screening of antibodies. Based on Sepharose™ with native protein A as ligand.

アプリケーション

Protein A Mag Sepharose™ Xtra magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants. The high capacity, > 27 mg human IgG/mL medium allow for the efficient capture of the antibody with high yield and purity. Approximately 350 μg purified human IgG can be obtained from each purification run.

Robust parallel screening of antibodies with high reproducibility.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device.

特徴および利点

  • Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
  • Optimized high capacity for efficient small-scale purification and screening of antibodies from various species
  • High purity and yield

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Sepharose is a trademark of Cytiva

ピクトグラム

Flame
GHS02

シグナルワード

Warning

危険有害性情報

H226

保管分類コード

3 - Flammable liquids

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試験成績書（COA）

Lot/Batch Number

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Lukas Leiendecker et al.
EMBO molecular medicine, 12(11), e12525-e12525 (2020-10-08)
Merkel cell carcinoma (MCC) is a highly aggressive, neuroendocrine skin cancer that lacks actionable mutations, which could be utilized for targeted therapies. Epigenetic regulators governing cell identity may represent unexplored therapeutic entry points. Here, we targeted epigenetic regulators in a
Qingzhou Ji et al.
Nucleic acids research, 42(10), 6158-6167 (2014-05-06)
Artificial transcription factors are powerful tools for regulating gene expression. Here we report results with engineered zinc-finger transcription factors (ZF-TFs) targeting four protein-coding genes, OCT4, SOX2, KLF4 and c-MYC, and one noncoding ribonucleic acid (RNA) gene, the microRNA (miRNA) miR302/367

資料

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

プロトコル

Purification of IgG Antibodies with Mag Sepharose®

Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら（テクニカルサービス）