おすすめの製品
ligand
glutathione
物質
polypropylene and polyethylene
メーカー/製品名
Cytiva 28-4055-00
包装
pkg of 4 x 96-well
サイズ
127.8 mm × 85.5 mm × 30.6 mm
Matrix
4% agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
4-13
動作範囲
4-13
キャパシティ
>25 mg binding capacity (horse liver GST/ml medium)
関連するカテゴリー
詳細
アプリケーション
Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap™ filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.
GST MultiTrap™ FF is prepacked with Glutathione Sepharose™ 4 Fast Flow, while GST MultiTrap™ 4B is prepacked with Glutathione Sepharose™ 4B.
特徴および利点
- 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
- Highly reproducible well-to well and plate-to-plate results.
- High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
- Wells are prepacked with Glutathione Sepharose™ media for convenience.
- Easy and predictable scale-up using prepacked GSTrap™ FF, GSTPrep™ FF 16/10, and GSTrap™ 4B columns.
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
本サイトでは、Cytiva™製品(日本国内取扱いなし)を用いたGST標識タンパク質の精製におけるトラブルシューティングの戦略について説明します。
プロトコル
This page shows how to solubilize membrane proteins with products from Cytiva.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら(テクニカルサービス)