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Merck

GE25-8010-65

Sigma-Aldrich

CyDye DIGE Fluor Minimal Labeling Kit

Cytiva 25-8010-65, pack of 5 nmol

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About This Item

UNSPSCコード:
12352200
NACRES:
NA.32

形状

solid

シェルフライフ

8‑12 mo. (for unreconstituted dye)

特徴

wetted part: no

包装

pack of 5 nmol

メーカー/製品名

Cytiva 25-8010-65

保管条件

protect from light

blue
red
yellow
Yellow, Red, Blue

溶解性

cold water: soluble (Soluble in cold water and hot water)
soluble (in cold and hot water)

輸送温度

dry ice

保管温度

−20°C

関連するカテゴリー

詳細

CyDye DIGE Fluor minimal dyes and saturation dyes are size- and charge-matched fluorescent dyes specifically designed for detecting protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis using Ettan DIGE system.

CyDye DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection. In total, three minimal dyes and two saturation dyes are available.

CyDye DIGE fluors are exceptional dyes for multicolor analysis, offering bright and intense colors with narrow excitation and emission bands. The fluors are spectrally distinct, making them highly suitable for multicolor detection. CyDye DIGE fluors utilize these benefits but are also size- and charge-matched specifically for 2-D DIGE using Ettan DIGE system.
fluorescence color: blue, green, red

The dyes are dissolved in Dimethylformamide (DMF) and the minimal labeling reaction is quenched with L-Lysine

特徴および利点

  • Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel.
  • Size- and charge-matched dyes enable co-migration of labeled samples within the gel.
  • Bright and highly sensitive dyes allow the use of the minimal labeling technique.
  • Minimal loss of signal during labeling, separation, and scanning.
  • No change in signal over wide pH range used during first-dimension (IEF) separation.
  • Discrete signal from each fluor with minimal cross-talk contributes to high accuracy.

構成

5 nmol of CyDye DIGE fluor minimal labeling kit (Cy2, Cy3 and Cy5)

注意

avoid light, store in the dark

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

CyDye:
Cy3B: This product is manufactured under an exclusive license from Carnegie Mellon University and is covered by US patent number 6,133,445 and equivalent patents and patent applications in other countries.
CyQ (or Cy5Q or Cy7Q): These products are covered under US patent number 6,828,116 and equivalent patents and patent applications in other countries in the name of Cytiva.
CytoCy5S: The use of this product in an NTR gene reporter assay is the subject of US patent number 7,579,140 in the name of Cytiva.
CypHer5E: This product or portions thereof is manufactured under license from Carnegie Mellon University and is covered by US patent number 7,615,646.
Cy and CyDye, are - trademarks of Cytiva or one of its subsidiaries.
The purchase of CyDye products includes a limited license to use the CyDye products for internal research and development but not for any commercial purposes. A license to use the Cy and CyDye trademarks for commercial purposes is subject to a separate license agreement with Cytiva. Commercial use shall include:
1. Sale, lease, license or other transfer of the material or any material derived or produced from it.
2. Sale, lease, license or other grant of rights to use this material or any material derived or produced from it.
3. Use of this material to perform services for a fee for third parties, including contract research and drug screening.
If you require a commercial license to use the Cy and CyDye trademarks please contact LSlicensing@cytiva.com.

If you require a commercial license to use this material and do not have one, return this material unopened to Cytiva Bio-Sciences AB, Bjorkgatan 30, SE-751 84 Uppsala, Sweden and any money paid for the material will be refunded.
CyDye is a trademark of Cytiva
Ettan is a trademark of Cytiva

ピクトグラム

Exclamation mark

シグナルワード

Danger

危険有害性情報

保管分類コード

13 - Non Combustible Solids


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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資料

Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.

プロトコル

This page describes two-dimensional separation of protein samples with Cytiva products.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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