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ligand
human IgG
包装
pack of 10 mL
メーカー/製品名
Cytiva 17-0969-01
保管条件
(20% Ethanol, 50 mM K2PO4, 1mM MgCl2 pH 7.2 in 20% Ethanol)
Matrix
highly cross-linked 6% agarose
平均直径
90 μm
cleaning in place
3-12
動作範囲
3-10
キャパシティ
~3 mg binding capacity(Protein A)
保管温度
2-8°C
関連するカテゴリー
詳細
lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. The improved mechanical characteristics of this Fast Flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
The mechanical characteristics of this fast flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
特徴および利点
- IgG coupled to the highly cross-linked 6% agarose matrix Sepharose™ 6 Fast Flow, designed for the rapid purification of protein A and protein A fusion conjugates
- Binds at least 2 mg protein A/mL medium
- Suitable for tandem affinity purification (TAP) of protein complexes
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
プロトコル
This page shows how to purify protein A-tagged proteins using Cytiva products.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
This page shows how to prepare samples for purification with affinity chromatography.
関連コンテンツ
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
親和性、GSTプルダウン、TAP、共免疫沈降法を利用して、プルダウンアッセイでin vitroタンパク質間相互作用を調査します。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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