おすすめの製品
包装
pack of 100 mL
メーカー/製品名
Cytiva 17-0920-07
Matrix
highly cross-linked 6% agarose
平均部品サイズ
34 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
動作範囲
3-12
キャパシティ
≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
詳細
アプリケーション
特徴および利点
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
- Conveniently charge with your metal of choice
- 34 μm bead size for high performance purification
- High binding capacity
保管および安定性
アナリシスノート
法的情報
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
How to optimize purification of histidine-tagged proteins using Cytiva products.
プロトコル
Protocol for Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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