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Merck

GE17-0920-07

IMAC Sepharose High Performance

Cytiva 17-0920-07, pack of 100 mL

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About This Item

UNSPSCコード:
41106500
NACRES:
NA.56
現在、価格および在庫状況を閲覧できません。

包装

pack of 100 mL

メーカー/製品名

Cytiva 17-0920-07

Matrix

highly cross-linked 6% agarose

平均部品サイズ

34 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

動作範囲

3-12

キャパシティ

≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

詳細

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

アプリケーション

Conveniently charge with your metal of choice. IMAC Sepharose FF is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2?, Zn2?, Ni2?, or Co2?); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose. Small bead size for high performance purification and high binding capacity.

特徴および利点

  • For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
  • Conveniently charge with your metal of choice
  • 34 μm bead size for high performance purification
  • High binding capacity

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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試験成績書(COA)

Lot/Batch Number

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資料

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

How to optimize purification of histidine-tagged proteins using Cytiva products.

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プロトコル

Protocol for Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

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