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ligand
sulphopropyl
詳細
Ion Exchanger Type (value)
包装
pack of 300 mL
メーカー/製品名
Cytiva 17-0729-01
Matrix
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
3-14
動作範囲
4-13
キャパシティ
70 mg binding capacity(ribonuclease A/ml medium)
適合性
suitable for bioprocess medium
関連するカテゴリー
詳細
SP Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with sulphopropyl (Q) strong cation exchange groups. SP Sepharose™ Fast Flow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with SP Sepharose™ Fast Flow is straight forward and the medium is available in a range of pre-packed process development tools.
As member of the BioProcess media range, SP Sepharose™ Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特徴および利点
- Well-proven strong cation exchanger developed for industrial downstream processes.
- Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
- The industry standard for ion exchange chromatography during recent decades
- High chemical stability allows for well proven CIP and sanitization protocols
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
プロトコル
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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