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UFC9100

Millipore

Amicon® Ultra Centrifugal Filter, 100 kDa MWCO

sample volume 15 mL, regenerated cellulose membrane, MWCO 100 kDa

別名:

100 kDa MWCO, 15 mL sample volume, Centrifugal concentrator

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About This Item

UNSPSCコード:
41104916
eCl@ss:
36100101
NACRES:
NB.24
物質:
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
ポアサイズ:
100 kDa MWCO
L:
12.1 cm (4.807 in.)
直径:
2.97 cm
ろ過面積:
7.6 cm2
容量:
15 mL
無菌性:
non-sterile
価格・在庫情報は確認できません。詳細については、Merckをご覧ください。

物質

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

品質水準

400

無菌性

non-sterile

製品種目

Amicon®

メーカー／製品名

Amicon® Ultra

パラメーター

15 mL sample volume

テクニック

protein extraction: suitable
protein purification: suitable

L

12.1 cm (4.807 in.)

直径

2.97 cm

ろ過面積

7.6 cm2

容量

15 mL

ポアサイズ

100 kDa MWCO

輸送温度

ambient

関連するカテゴリー

詳細

Amicon® Ultra 15 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >90% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.

アプリケーション

  • Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.

特徴および利点

  • Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipettor sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

法的情報

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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資料

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

ナノ粒子の限外ろ過

ナノ粒子の精製と製造における、ろ過法とメンブレンフィルターの選定。

タンパク質サンプルの限外ろ過

このページでは、タンパク質サンプル調製に限外ろ過膜を使用することの原理と利点を考察しています。用途には溶媒交換、タンパク質サンプルの濃縮、サンプルの精製などが考えられます。

Protein Sample Ultrafiltration

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

関連コンテンツ

Amicon® Ultra Centrifugal Filters

Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.

質問

1–10/12 質問  

  1. Hello, Are these tubes valid for AAV concentration? And if so, are these valid for all serotypes? Also, the user guide says that the membrane's material is 'Ultracel® low binding regenerated cellulose'. What's the difference with the PES membrane?

    1 回答

    1. These devices can be used for virus concentration but are not validated for any specific type. Both PES and regenerated cellulose membranes have low binding, but PES has a faster flow rate. For more information, please refer to the technical article on the website: https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/viral-concentration-amicon-ultrafiltration

      役に立ちましたか？

  2. Hi, I have plan to use the Amicon Ultra-15 100K for Exosome extraction from cell culture supernatant, could you please let me know what would be the best speed (rpm/rcf) and time for doing that? Thanks

    1 回答

    1. Typically, Amicon-15 devices are spun at 4,000 x g (relative centrifugal force) for exosome isolation. However, spin times may need to be optimized, based on the sample type and volume. The image below demonstrated the relationship between volume and spin times. Please see the link below to review the product User Guide. More specific spin time information is available on page 3.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdf

      役に立ちましたか？

  3. What is the minimum starting volume I can load on Ultra 100K (100,000 MWCO) devices? Can I load as little as 500 µL?

    1 回答

    1. This product is intended for use with 4-15 mL initial sample volumes. The device has not been evaluated for use with volumes smaller than those recommended. While it may be possible to concentrate samples as low as 500 uL, the recovery volume cannot be predicted. Please see the link below to review an alternative Amicon device specifically designed for 500 uL samples.
      https://www.sigmaaldrich.com/product/mm/ufc5100

      Please see the following link to review the full list of 100 kDa MWCO Amicon Ultra products:
      https://www.sigmaaldrich.com/substance/amiconultracentrifugalfilter100kdamwco1234598765

      役に立ちましたか？

  4. Does the Amicon Ultra has residues that may released into the concentrated product', like Beta-Glucan?

    1 回答

    1. These devices contain trace amounts of glycerine. If this material interferes with downstream analysis, rinse the device with buffer or Milli-Q® water prior to use. Should interference continue, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q® water. NOTE: Do not allow the membrane to dry out following rinse steps. If the product will not be used immediately after rinsing, leave the final buffer or water on the membrane until the usage. Please see the link below to review the full product User Guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdf

      役に立ちましたか？

  5. I just noticed that Amicon ultra - 15 centrifugal filters are non-sterile. Has this been always the case? REF: UFC910024, LOT:0000193199

    1 回答

    1. The Amicon Ultra devices have always been non-sterile. These devices cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:

      1. Reagent preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter devices, such as the 50 mL Steriflip® device SCGP00525 or SE1M179M6.

      2.Sanitization Protocol:

      A. Fill the Amicon® Ultra concentrator device to its published capacity with filter-sterilized 70% Ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).
      B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.
      C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).
      D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.
      E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.
      F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.
      G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.

      DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from device as previously described prior to use.

      役に立ちましたか？

  6. What is the maximum volume that can be loaded into the Amicon 15 device when using both fixed angle and swinging bucket rotor centrifuges?

    1 回答

    1. When using the fixed angle centrifuges with the Amicon 15 device, the maximum volume that can be loaded is 12 mL. It is important to place the device at a 35-degree angle. On the other hand, when using swinging bucket centrifuges, the maximum volume that can be loaded into the Amicon 15 device is 15 mL.

      役に立ちましたか？

  7. Hi, how should we place the amicon ultra-15 tube in the fixed-angle rotor, (could you please explain the sentence "When using a fixed-angle rotor, orient the device with the membrane panel facing up" , sorry I'm not a native English speaker

    1 回答

    1. When using a fixed-angle rotor, orient the device with the membrane panel facing up as demonstrated in the image below.

      For a more detailed view, kindly review the attached video link: https://youtu.be/GTH7kQMUjFg?feature=shared&t=145. This video also discusses the relative size of each Amicon® Ultra Centrifugal Filter and what kind of rotors they will fit into.

      役に立ちましたか？

  8. Hii, I wold like use these tubes for ultrafiltration of FBS to deplete it from EVs. I found they were used in one publication for such an experiment. How can they be sterilized so they can be used in cell culture hoods?

    1 回答

    1. Amicon Ultra devices cannot be sterilized by UV or autoclaving. These products are intended as a 1 time use device, however it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:

      1. Reagent preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter devices, such as the 50 mL Steriflip® device SCGP00525 or SE1M179M6.

      2.Sanitization Protocol:

      A. Fill the Amicon® Ultra concentrator device to its published capacity with filter-sterilized 70% Ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).

      B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.

      C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).

      D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.

      E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.

      F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.

      G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.

      DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from the device as previously described prior to use.

      役に立ちましたか？

  9. Hi, Can you please confirm the centrifugation speeds ( in rcf ) for the Amicon-15 100kDa and the 10kDa filters ? I was told that the max speed for the 100kDa is 3000g and for the 10kDa is 4000g, respectively. Is that the case and if so, why exactly ?

    1 回答

    1. Spin times range from 10 - 40 minutes and will depend on the sample types as well as the rotor (fixed-angle or swinging bucket). See the image below for spin times and speeds for each rotor type.
      Please see the link below to review the product FAQs for additional usage information:
      https://www.emdmillipore.com/US/en/product/Amicon-Ultra-15-Centrifugal-Filter-Unit,MM_NF-UFC910008#documentation

      To request the product user guide, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Documentation" under the Products Section with all the required information so that a member of our team can reach out to you to assist further.

      役に立ちましたか？

  10. Please could you provide the manual for these kind of filters: Amicon Ultra 15 ml centrifugal filters

    1 回答

    1. Kindly review the user guide for these products at the following link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/360/134/pr05035-rev1018.pdf

      役に立ちましたか？

1–10/12 質問  

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