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安全性情報

GAHK20

Supelco

グルコース(HK)アッセイキット

sufficient for 20 assays

別名:

グルコース定量キット

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1 KIT
￥15,300

￥15,300

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1 KIT
￥15,300

About This Item

UNSPSCコード:
12164500
NACRES:
NA.84

￥15,300

Check Cart for Availability
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使用法

sufficient for 20 assays

アナライトの化学物質クラス

sugars (glucose)

テクニック

photometry: suitable

アプリケーション

food and beverages
general analytical

保管温度

2-8°C

関連するカテゴリー

アプリケーション

本キットは、食物および他の材料中のグルコ-スを定量的、酵素的に測定するための製品です。グルコ-スは、ヘキソキナ-ゼが触媒する反応においてアデノシン三リン酸 (ATP) によりリン酸化されます。次に、グルコ-ス 6-リン酸(G6P)は、酸化型ニコチンアミドアデニンジヌクレオチド (NAD) の存在下で グルコ-ス 6-リン酸デヒドロゲナ-ゼ (G-6PDH)が触媒する反応において酸化されて6-ホスホグルコン酸になります。この酸化の間に、等モルのNADが還元されて NADHになります。その結果として生ずる 340 nmでの吸光度の増加は、グルコ-スの濃度に正比例します。

キットの構成要素のみ

製品番号
詳細
  • glucose (HK) assay reagent 20 mL
  • glucose standard .5 mL

保管分類コード

10 - Combustible liquids

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

毒物及び劇物取締法

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PRTR

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消防法

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カルタヘナ法

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Jan Code

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最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
0000411005
0000411005
0000331189
0000351488
0000294939

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特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Rebecca A Haeusler et al.
Nature communications, 5, 5190-5190 (2014-10-14)
Insulin integrates hepatic glucose and lipid metabolism, directing nutrients to storage as glycogen and triglyceride. In type 2 diabetes, levels of the former are low and the latter are exaggerated, posing a pathophysiologic and therapeutic conundrum. A branching model of
Kuei-Ching Hsiung et al.
Biomedical journal, 43(1), 32-43 (2020-03-24)
CDGSH iron sulfur domain-containing protein 1 (CISD-1) belongs to the CISD protein family that is evolutionary conserved across different species. In mammals, CISD-1 protein has been implicated in diseases such as cancers and diabetes. As a tractable model organism to
Bo-Ruei Chen et al.
Experimental gerontology, 44(8), 493-502 (2009-05-05)
We describe a new chronological lifespan (CLS) assay for the yeast Schizosaccharomyces pombe. Yeast CLS assays monitor the loss of cell viability in a culture over time, and this new assay shows a continuous decline in viability without detectable regrowth
Louis-Philippe Bernier et al.
Nature communications, 11(1), 1559-1559 (2020-03-28)
Microglia are highly motile cells that continuously monitor the brain environment and respond to damage-associated cues. While glucose is the main energy substrate used by neurons in the brain, the nutrients metabolized by microglia to support surveillance of the parenchyma
Saheed Imam et al.
BMC systems biology, 5, 116-116 (2011-07-23)
Rhodobacter sphaeroides is one of the best studied purple non-sulfur photosynthetic bacteria and serves as an excellent model for the study of photosynthesis and the metabolic capabilities of this and related facultative organisms. The ability of R. sphaeroides to produce
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質問

1–10/13 質問  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      役に立ちましたか？

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 回答

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      役に立ちましたか？

  3. The procedure says that solutions that are strongly colored should be decolorized for the kit to work. Do you have any suggestions on how to decolorize my sample?

    1 回答

    1. Solutions may be decolorized by adding a small amount of charcoal, stirring a few moments, and then clarifying by filtration or centrifugation to remove the charcoal. An activated charcoal product, such as item 161551 would be appropriate. See the link below to review this product:
      https://www.sigmaaldrich.com/product/sigald/161551

      役に立ちましたか？

  4. I want to detect the change of glucose concentration in macrophages. How to prepare the sample

    1 回答

    1. A macrophage sample should be lysed using a cell lysis protocol similar to one used for Western Blotting. A useful lysis protocol can be found linked below.

      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/western-blotting/sample-preparation

      Following cell lysis, the sample should be diluted with deionized water to 0.05 to 5 mg of glucose per mL. The sample should be deproteinized (using a common perchloric acid precipitation procedure) and filtered to clarify the solution. Any solutions that are strongly colored should be decolorized using a decolorization agent such as activated charcoal. Any samples in which gases are liberated during the sample preparation process should be degassed prior to analysis.

      役に立ちましたか？

  5. How to adapt the Glucose Assay Kit to a 96-well plate?

    1 回答

    1. It may be possible to adapt this kit for use in a 96-well plate format. This would require method development and reagent scale down by the end user. There are no specific instructions available. Should a 96-well assay be preferred or required, please see product MAK476 at the link below. This product has been optimized for use in this format.
      https://www.sigmaaldrich.com/product/sigma/mak476

      役に立ちましたか？

  6. Is the product G3293-20ML included in the GAHK20-1KT kit?

    1 回答

    1. Yes, the item G3293-20ML is a component of the complete GAHK20-1KT kit. Therefore, if G3293-20ML is on backorder, it can be obtained by ordering the GAHK20-1KT.

      役に立ちましたか？

  7. Is GAHK20 assay compatible with C. elegans worm lysates? What buffer you recommed to prepare lysates?

    1 回答

    1. The Glucose (HK) Assay Kit has been optimized for use in food and other crude materials. This kit has not been qualified for use with cells or organisms such as C. elegans. The researcher would have to determine suitability, however there is some indication that other customers have been successful. See the link below for a publication that may be of interest:
      https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1005561&type=printable

      Note that samples may require centrifugation, filtration or deproteinization to further clarify.

      役に立ちましたか？

  8. Can the procedure for Glucose (HK) Assay Kit, Product GAHK20, be scaled down?

    1 回答

    1. Although we have not done it, you should be able to scale down the procedure as long as you have pipettes capable of dispensing accurate ul volume quantities and an instrument that can monitor the 340 nm wavelength.

      役に立ちましたか？

  9. How do I determine what sample size to use in the Glucose (HK) Assay Kit, Product GAHK20?

    1 回答

    1. If you know approximately how much glucose is present, dilute the sample down to 0.5 to 50 ug of glucose and use between 10 to 200 ul sample volume.  If you do not know how much glucose is present, you should assay the undiluted sample and dilutions of it.  Based on the dilution factor you can determine the glucose concentration.

      役に立ちましたか？

  10. How do I decolorize a solution for use with Product GAHK20, Glucose (HK) Assay Kit?

    1 回答

    1. You can decolorize a solution by adding a small amount of charcoal to the solution, swirl, then filter or centrifuge to remove charcoal.

      役に立ちましたか？

1–10/13 質問  

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