immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10,000 using rat brain sections microarray: suitable western blot: 1:10,000 using rat brain cytosolic fraction
While glutamine is a nonessential amino acid that can be synthesized from glucose, some cancer cells primarily depend on glutamine for their growth, proliferation, and survival. Numerous types of cancer also depend on asparagine for cell proliferation. The underlying mechanisms
High-Fat Diet (HFD)-induced obesity may promote agerelated memory impairment via disturbances of ammonia-glutamine metabolism. We studied the effects of age and long-term HFD exposure on Glutamine Synthetase (GS) expression in the liver and hippocampus and recognition memory in mice. Adult
Primary culture of trigeminal satellite glial cells: a cell-based platform to study morphology and function of peripheral glia
Poulsen JN, et al.
International Journal of Physiology, Pathophysiology and Pharmacology, 6(1), 1-1 (2014)
Small guanosine triphosphatase (GTPase) ADP-ribosylation factors (Arfs) regulate membrane traffic and actin reorganization under the control of GTPase-activating proteins (GAPs). Arap1 is an Arf-directed GAP that inhibits the trafficking of epidermal growth factor receptor (EGFR) to the early endosome, but