For a list of components, see the link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/747/307/d8437for.pdf
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製品名
ダルベッコ改変イーグル培地/栄養混合物F-12ハム, With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
品質水準
無菌性
sterile-filtered
フォーム
liquid
テクニック
cell culture | mammalian: suitable
不純物
endotoxin, tested
構成
glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L
輸送温度
ambient
保管温度
2-8°C
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詳細
ダルベッコ改変イーグル培地（DMEM）′′は、よく使用される細胞培地であり、グルコース、ビタミン、アミノ酸が含まれるエネルギー豊富な培地の供給源です。[1]ダルベッコ改変イーグル培地（DME）とハムF-12栄養混合物の1：1の混合物は、細胞培養研究で広く使用されています。′′′血清を除外したことによる緩衝能の喪失を補うため、4-(2-ヒドロキシエチル)-1-ピペラジンエタンスルホンさん（HEPES）バッファーを最終濃度が15 mMになるように培地組成に添加することができます。
アプリケーション
よく一緒に購入される製品
製品番号
詳細
価格
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
D8437-6X500ML:
D8437-500ML:
D8437-BULK:
D8437-VAR:
D8437-24X500ML:
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資料
Nutrient Mixture F-12 Ham Formulation
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Dear, Can you tell me the composition of this medium, or at least the amount of L-Glutamine present in this media? Kind regards, Sarah
1 回答-
役に立ちましたか？
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Is the glutamine that has already been added stable glutamine? How long is the shelf life?
1 回答-
This product does not contain Alanyl-glutamine, which is considered a more stable form It has been supplemented with L-glutamine and will remain stable through out the shelf life of the product with proper storage and handling. Typical lots of D8437 are assigned an expiration date of 1 year from the date of release. Note that under culture conditions of 37 deg C the L-glutamine will gradually degrade. See the link below to review general stability information regarding L-glutamine.:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/195/924/t075.pdfAlanyl-glutamine is also available as a sterile medium supplement. Please see the link below for an example of one cell culture product option:
https://www.sigmaaldrich.com/product/sigma/g8541役に立ちましたか？
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Can Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, be used under serum free conditions?
1 回答-
When DME/F12 is used under serum free conditions, supplementation is necessary. DME/F12 can be used for growing the epithelial cell line TM4 (mouse testes) when supplemented with insulin, transferrin, EGF, FSH, somatomedin C, ovine growth hormone, and retinoic acid. Primary cultures of Sertoli cells can also be grown in the above supplemented media. Rat glial C6 cells have been grown in DME/F12 supplemented with transferrin, insulin, LH, and rat submaxillary gland extract. See: Jakoby, W.B., and Pastan, I.H., Cell Culture, Methods in Enzymology, 58, 103-106 (1979). (This book is listed as Product No. C0541).
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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What is the optimal carbon dioxide concentration needed for Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
1 回答-
This is a 1:1 mixture of the DME and F-12. The optimal carbon dioxide required for DME is 10% and for F-12 is 5%. Since this medium is a mixture, the optimal carbon dioxide concentration is typically 5% to 8%.
役に立ちましたか？
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What is the storage temperature of Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
1 回答-
We recommend storing Product No. D8437 at 2-8°C.
役に立ちましたか？
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What is Product D8437, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham?
1 回答-
This product is a 1:1 ratio mixture of Dulbecco’s Modified Eagle’s Medium (DME) high glucose and Nutrient Mixture F-12 Ham (F-12).
役に立ちましたか？
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