D7440

JumpStart^™Taq ReadyMix^™ 定量PCR用

For probe-based real-time PCR

• 別名: hot start DNA polymerase, hot start PCR, hot start master mix, qPCR, real time quantitative PCR
• UNSPSCコード: 41106300
• NACRES: NA.55

特性

• 品質水準: 200
• フォーム: liquid
• 使用法: sufficient for 100 reactions; sufficient for 20 reactions; sufficient for 400 reactions
• 特徴: Multiplex PCR; dNTPs included; hotstart
• 濃度: 2.5 units/reaction (50 μL reaction volume)
• テクニック: qPCR: suitable
• 色: colorless
• 入力: purified DNA
• アプリケーション: agriculture
• 適合性: Bio-Rad MyiQ ( ); Bio-Rad iCycler iQ; Bio-Rad iQ 5; for use with ABI 5700; for use with ABI 7000; for use with ABI 7300; for use with ABI 7500 Fast; for use with ABI 7500; for use with ABI 7700; for use with ABI 7900 Fast; for use with ABI 7900 HT; for use with ABI 7900; for use with ABI StepOne; for use with ABI StepOnePlus; for use with ABI ViiA 7; for use with Bio-Rad CFX384; for use with Bio-Rad CFX96; for use with Bio-Rad MJ Chromo4; for use with Bio-Rad MJ Opticon 2; for use with Bio-Rad MJ Opticon Cepheid SmartCycler; for use with Bio-Rad MJ Opticon; for use with Bio-Rad MiniOpticon; for use with Eppendorf^® Mastercycler ep realplex2 s; for use with Eppendorf^® Mastercycler ep realplex; for use with Illumina Eco qPCR; for use with Qiagen Corbett Rotor-Gene 3000; for use with Qiagen Corbett Rotor-Gene 6000; for use with Qiagen Corbett Rotor-Gene Q; for use with Roche LightCycler 480; for use with Strategene Mx3000P; for use with Strategene Mx3005P; for use with Strategene Mx4000
• 検出方法: probe-based
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

JumpStart^™ Taq ReadyMix^™ is a ready-to-use 2X master mix that contains JumpStart^™ Taq DNA polymerase, 99% pure dNTPs, reaction buffer and JumpStart^™ Taq antibody. It combines the performance enhancements of our JumpStart^™ Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture. Since it has no added dyes, this is the ideal solution for performing high-throughput, quantitative PCR methods that rely on a fluorescent probe. The Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

アプリケーション

JumpStart^™ Taq ReadyMix^™ for Quantitative PCR has been used:

• for quantitative polymerase chain reaction (qPCR) amplification of HIV-1 RNA that is reverse transcribed and DNA using probes[1][2]
• in a 2-step RT-qPCR assay for the quantification of reverse transcribed RNA in a study to monitor the activity of licorice in Treg cell differentiation and function[3]
• as a component of the reaction mixture for the detection and amplification of Salmonella sp. and internal amplification control (IAC) pUC 19 plasmid DNA[4]
• as a component of the reaction mixture for the detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)[5]
• for quantitative real-time -PCR of reverse-transcribed ß-arrestin-1 (ARRB1) and β-arrestin-2 (ARRB2) mRNA[6]

特徴および利点

• Ultimate Convenience: ReadyMixes contain all components necessary for QPCR, simply add fluorescent detection chemistry, primers, and template
• Greater Specificity & Increased Target Yield: JumpStart^™ Taq prevents non-specific amplification and increased target yield
• Maximum Flexibility: Suitable for use with a variety of detection chemistries including molecular probes and double-stranded binding dyes such as SYBR^® Green I
• Designed for use with either plate/tube real-time thermal cyclers or capillary instruments

包装

Sigma′s Reference Dye for Quantitative PCR is included separately with this ReadyMix for normalization of the reaction data. The dye has a maximum excitation of 586 nm, and a maximum emission of 605 nm. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. A tube of 25 mM MgCl₂ is also provided for easy optimization of the qPCR reaction.

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL

その他情報

JumpStart Taq ReadyMix for Quantitative PCR combines the advantages of a hot start enzyme with a ready-to-use mix for high throughput, quantitative PCR (qPCR). It is formulated without a detection chemistry making it suitable for use with a variety of formats including dual-labeled probes, molecular beacons, or double stranded binding dyes such as SYBR Green I.

原理

The ReadyMix contains JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, and buffer in an optimized 2× concentrate. To prepare a reaction, 25 μL of the ReadyMix is added to primers, template, detection chemistry, and water for a total reaction volume of 50 μL. Set up can be performed at room temperature since the JumpStart Taq antibody renders the Taq DNA polymerase inactive. During the first denaturation cycle, the antibody dissociates from the enzyme and full activity is restored. No special preparations or protocol changes are required.

単位の定義

1 unitは、74°C、30分間に、10 nmolの全dNTPを酸不溶性DNAに取り込む酵素量です。

法的情報

(i)認可された5' Nuclease Core Kitとライセンスを受けたプローブを両方購入した場合、(ii)ライセンスを受けた5' Nuclease Kitを購入した場合、(iii)Applied Biosystemsからライセンス権を購入した場合、特許で保護されている5' Nuclease Processを研究で使用するライセンスが得られます。

本製品は、認可された5' Nuclease Core Kitです。本製品を使用する場合、米国特許番号 5,210,015、5,487,972と、対応する米国外での特許請求のうち、1つ以上が適用されます。本製品の購入について、購入者自身の内部研究のために購入した量を使用する場合に限り、前述の特許請求に基づく訴訟から免責されますが、これは限定的なものであり、譲渡することはできません。ライセンスを受けたプローブを別途購入することによって、米国特許番号5,538,848、5,723,591、5,876,930、6,030,787、6,258,569、5,804,375(請求項1～12のみ)、対応する米国外の特許請求、米国特許番号6,214,979に対応する米国外請求に基づく権利が譲渡されます。他の特許によって保護されている権利や、あらゆる種類の商業サービス(購入者の研究結果を報酬やその他の商業的事由のために無制限に報告することを含む)を行う権利が、暗示や禁反言によって明示的に譲渡されるわけではありません。本製品は研究専用です。Rocheの特許に基づき診断に利用する場合は、別途Rocheからライセンスを受ける必要があります。ライセンス購入に関する詳細な情報については、ライセンス管理者(Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA)にお問い合わせ下さい。

Eppendorf is a registered trademark of Eppendorf AG

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

キットの構成要素は別途購入可能です。

• P2893 JumpStart^™ Taq ReadyMix^™, Complete optimized reagent for hot-start PCR at 2X concentration 2 X
• M8787 25 mM MgCl₂ 1.5 μL
• R4526 Reference Dye for Quantitative PCR, 100 ×, solution 100 X

安全性情報

• ピクトグラム: GHS07,GHS09
• シグナルワード: Warning
• 危険有害性情報: H315,H317,H319,H410
• 注意書き: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• 危険有害性の分類: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• 保管分類コード: 12 - Non Combustible Liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable