コンテンツへスキップ
Merck
すべての画像(1)

主要文書

安全性情報

D2189

Sigma-Aldrich

[Glu52]-ジフテリア毒素 ジフテリア菌由来

lyophilized powder

別名:

CRM 197

ログイン組織・契約価格を表示する


About This Item

CAS番号:
UNSPSCコード:
12352202

フォーム

lyophilized powder

品質水準

保管温度

2-8°C

類似した製品をお探しですか? 訪問 製品比較ガイド

包装

包装サイズはタンパク質含量を基準にしています。

物理的形状

Lyophilized powder containing sodium phosphate, sucrose and polysorbate 80

保管分類コード

11 - Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, type N95 (US)


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

D2189-500UG:
D2189-BULK:
D2189-250UG:
D2189-1MG-PW:
D2189-1MG:
D2189-VAR:


最新バージョンのいずれかを選択してください:

試験成績書(COA)

Lot/Batch Number

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Naoko Kunami et al.
Anticancer research, 31(7), 2483-2488 (2011-08-30)
The therapeutic outcome for T-cell acute lymphoblastic leukemia (T-ALL) remains poor; thus, novel, targeted therapies are urgently needed. Recently, we showed that heparin-binding epidermal growth factor-like growth factor (HB-EGF), a member of the EGF family, is a promising target for
Enrico Malito et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(14), 5229-5234 (2012-03-21)
CRM197 is an enzymatically inactive and nontoxic form of diphtheria toxin that contains a single amino acid substitution (G52E). Being naturally nontoxic, CRM197 is an ideal carrier protein for conjugate vaccines against encapsulated bacteria and is currently used to vaccinate
Fabio Fiorino et al.
Vaccine, 30(43), 6111-6114 (2012-06-19)
Typhoid fever is a public health problem, especially among young children in developing countries. To address this need, a glycoconjugate vaccine Vi-CRM₁₉₇, composed of the polysaccharide antigen Vi covalently conjugated to the non-toxic mutant of diphtheria toxin CRM₁₉₇, is under
F Micoli et al.
Vaccine, 30(5), 853-861 (2011-12-17)
A conjugate vaccine for Salmonella enterica serovar Typhi was produced by chemically linking Vi, purified from Citrobacter, to the non-toxic mutant diphtheria toxin CRM(197) via an adipic dihydrazide spacer using N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide coupling chemistry. The polysaccharide purification process was developed based
Matthias A Oberli et al.
Chemistry & biology, 18(5), 580-588 (2011-05-26)
Nosocomial infections with the Gram-positive pathogen Clostridium difficile pose a major risk for hospitalized patients and result in significant costs to health care systems. Here, we present the chemical synthesis of a PS-II hapten of a cell wall polysaccharide of

質問

1–4/4 質問  
  1. How do I reconstitute Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?

    1 回答
    1. The vial can be reconstituted with 1.0 mL of sterile water. Each vial contains 1 mg of Diphtheria toxin CRM mutant, sodium phosphate buffer, and lactose. It can sometimes be hard to get the product into solution due to the lactose used as a stabilizer. The supplier suggests slight warming (to at least room temperature or 30 °C, but not more than 37 °C) followed by a brief vortex to break up the filaments.

      役に立ちましたか?

  2. What is the Department of Transportation shipping information for this product?

    1 回答
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      役に立ちましたか?

  3. What is the molecular weight of Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?

    1 回答
    1. On reducing SDS gels, this protein migrates as a single band of an approximate molecular weight of 63,000 Daltons.

      役に立ちましたか?

  4. What kind of mutant, is Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae, and how is this product different from Product No. D0564?

    1 回答
    1. Product No. D0564 is the wild-type toxin and possesses enzymatic activity.  The Glu52 diptheria toxin, also known as CRM197, has a mutation in fragment A (a glycine to glutamic acid substitution at position 52) that results in the complete loss of enzymatic activity. More information on the mutant can be found in the following reference: Uchida, T., Pappenheimer, Jr., A.M. and Harper, A.A.,  J. Biol. Chem., 248, 3851-3854 (1973).

      役に立ちましたか?

レビュー

評価値なし

アクティブなフィルタ

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)