This kit has not been tested for use with confocal or fluorescent microscopes. X-gal staining does not involve a specific wavelength and it would be up to the end user to determine suitability. Note below, in a previously submitted question, that this kit has not been tested for dual staining with fluorescent dyes.
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使用法
sufficient for 100 tests
品質水準
包装
pkg of 1 kit
保管条件
dry at room temperature
検出方法
colorimetric
輸送温度
dry ice
保管温度
−20°C
詳細
老化細胞組織染色キットには、pH 6でのß-ガラクトシダーゼ活性の組織染色に基づくアッセイを用いて老化細胞を識別するために必要なすべての試薬が含まれています。ß-ガラクトシダーゼ活性は老化細胞で検出できますが、静止細胞、不死細胞や腫瘍細胞では検出できません。
老化細胞組織染色キットには、pH 6でのβ-ガラクトシダーゼ活性の組織化学染色に基づくアッセイを用いて老化細胞を識別するために必要なすべての試薬が含まれています。β-ガラクトシダーゼ活性は老化細胞で検出できますが、静止細胞、不死細胞や腫瘍細胞では検出できません。細胞の老化は、細胞が活発に分裂している段階から分裂していない段階に移行する事象の進行です。細胞の老化プロセスは加齢と関連しています。細胞分裂の減少は事実上不可逆的であり、完全です。分裂能の喪失とともに、細胞の形態、形状、外観、および遺伝子発現パターンに変化が生じます。このプロセスの終了時には通常、細胞死が起こりますが、細胞は長期間生存している可能性があります。
アプリケーション
老化細胞組織染色キットは、以下の用途に使用されています：
- 老化関連β-ガラクトシダーゼアッセイ
- ヒト膵がん細胞における老化の検証
- 脂肪由来幹細胞（ADSC）
- 臍帯血間葉系間質／幹細胞（CBMSC）
生物化学的／生理学的作用
複製老化は、分裂能の喪失、細胞の形態や形状、外見的な変化、遺伝子発現のパタ-ン変化に伴っておこる増殖の停止状態です。
法的情報
米国特許番号5,491,069および5,795,728のライセンスに基づいて販売しています。
キットの構成要素のみ
製品番号
詳細
- X-gal solution 4 mL
- Staining Solution, 10X 15 mL
- Fixation Buffer 10x 15 mL
- Reagent B 1.5 mL
- Reagent C 1.5 mL
- Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL
シグナルワード
Danger
危険有害性の分類
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
ターゲットの組織
Respiratory system
補足的ハザード
保管分類コード
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
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PRTR
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消防法
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労働安全衛生法名称等を表示すべき危険物及び有害物
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労働安全衛生法名称等を通知すべき危険物及び有害物
キットコンポーネントの情報を参照してください
カルタヘナ法
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Jan Code
キットコンポーネントの情報を参照してください
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
この製品を見ている人はこちらもチェック
Michael J MacLeod et al.
Journal of fish diseases, 41(9), 1359-1372 (2018-06-09)
In vitro cell culture methods are crucial for the isolation, purification and mass propagation of intracellular pathogens of aquatic organisms. Cell culture infection models can yield insights into infection mechanisms, aid in developing methods for disease mitigation and prevention, and
G P Dimri et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(20), 9363-9367 (1995-09-26)
Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions. This process, termed replicative senescence, is thought to be a tumor-suppressive mechanism and an underlying cause of aging. There is
B van der Loo et al.
Experimental cell research, 241(2), 309-315 (1998-06-25)
A beta-galactosidase activity has recently been used as a histochemical marker of replicative senescence in human fibroblasts and keratinocytes. To establish whether this marker could be used to detect senescence of vascular cells, we have investigated its presence in cultures
Mandy Oj Grootaert et al.
Autophagy, 11(11), 2014-2032 (2015-09-24)
Autophagy is triggered in vascular smooth muscle cells (VSMCs) of diseased arterial vessels. However, the role of VSMC autophagy in cardiovascular disease is poorly understood. Therefore, we investigated the effect of defective autophagy on VSMC survival and phenotype and its
Natalja E Fedorovich et al.
Biomaterials, 30(3), 344-353 (2008-10-22)
Photopolymerizable hydrogels, formed by UV-exposure of photosensitive polymers in the presence of photoinitiators, are widely used materials in tissue engineering research employed for cellular entrapment and patterning. During photopolymerization, the entrapped cells are directly exposed to polymer and photoinitiator molecules.
資料
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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Can we use confocal/basic fluorescent microscope for capturing the expression of Beta Galactosidase after using this staining kit?
1 回答-
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Is there a method to extract the stain from cells when using the Senescence Cells Histochemical Staining Kit (Catalog Number CS0030) for cells in transwell inserts where cell counting is not feasible?
1 回答-
The CS0030 kit is intended for cell visualization using a microscope and does not include a specific procedure for dye extraction from cells.
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Is there a protocol for staining HCHO-fixed tissue culture cells, specifically for cells growing on coverslips or slides?
1 回答-
For staining purposes, there is no significant difference between staining wells attached to a culture plate or cells attached to a coverslip or glass slide. The primary difference lies in the amount of reagent required for the staining. It is advisable to ensure adequate coverage of the cells, and the insert contains a table that provides guidance on the amount of reagent required for staining.
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Does kit CS0030 include a counterstain?
1 回答-
No, the CS0030 kit does not include a counterstain.
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I have been using it well for a long time. thank you I want to stain beta-gal staining and apoptosis-related IF together. Is there a recommended order or method?
1 回答-
The CS0030 kit has not been tested for use in dual staining, it would be up to the end user to determine suitability. There are several factors to consider with regard to attempting this modification.
1. The CS0030 includes a fixation step. Cells are not viable at the time of staining. Therefore any 'live cell' fluorescent stain would not be suitable following fixation.
2. There is an incubation period of 2 hours to overnight. This may deplete or cause certain fluorescent dyes to fade which would mean a 'one-time use' for visualizing the IF-stained cells.
3. The staining mechanism for CS0030 is pH dependent. This may not be suitable for some fluorescent dyes.
4. One possible robust fluorescent stain to consider would be DAPI, which could be applied following the b-gal staining. DAPI will stain fixed cells and can be preserved if stored in the dark.To discuss further, please navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquiries" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further.
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Can Product CS0030, Senescence Cells Histochemical Staining Kit be used with fixed cells/tissues?
1 回答-
Product CS0030, Senescence Cells Histochemical Staining Kit was designed for use with fresh (not-fixed) cells. The kit contains a fixation buffer to fix the cells. It is 10% formaldehyde, 1% glutaraldehyde in DPBS at 1X. The cells will be fixed prior to staining. The staining will show the beta-galactosidase activity that was present at the time of fixation.We tested the kit only on cells and we have no experience with tissue sections. The following papers use an assay for ßGAL on tissue sections which suggests that the kit will work with tissue sections.Castro P, et al., Interleukin-8 expression is increased in senescent prostatic epithelial cells and promotes the development of benign prostatic hyperplasia. Prostate, 60(2), 153-159 (2004).Chen, Z., et al., Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis. Nature, 436, 725-730 (2005).
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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