Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
￥86,500
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使用法
sufficient for 200 colorimetric test
輸送温度
dry ice
保管温度
−20°C
関連するカテゴリー
詳細
The detection range: 10-600 units/liter of Acetylcholinesterase activity.
The detection time: 5-10 minutes
Acetylcholinesterase ( AChE) is a serine hydrolase found at neuromuscular junctions and cholinergic brain synapses. AChE, hydrolyzes choline esters. Acethlcholinesterase activity is related to the function of the nerve response. Its principal biological role is the termination of impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) to acetate and choline.
Organophosphorus (OP) poisons form a covalent bond with a serine residue at the active site of AChE, and are thus potent irreversible inhibitors of AChE.
AChE inhibitors are used in treatment of various neuromuscular disorders, and have provided the first generation of drugs for the treatment of Alzheimer′s disease.
AChE inhibitors inhibit the cholinesterase enzyme from breaking down ACh, increasing both the level and duration of the neurotransmitter action According to the mode of action, AChE inhibitors can be divided into two groups: irreversible and reversible. Reversible inhibitors, competitive or noncompetitive, mostly have therapeutic applications, while toxic effects are associated with irreversible AChE activity modulators.
アプリケーション
Enables to screen for Acetylcholinesterase inhibitors
特徴および利点
- A simple, quick, sensitive, and direct procedure for measuring Acetylcholinesterase Activity levels in a variety of samples such as serum and plasma, tissues or cell lysates enables to screen for Acetylcholinesterase inhibitors
- Supportive calculator (Click here to download a calculator excel file): Will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
単位の定義
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Eye Dam. 1
保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
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PRTR
キットコンポーネントの情報を参照してください
消防法
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労働安全衛生法名称等を表示すべき危険物及び有害物
キットコンポーネントの情報を参照してください
労働安全衛生法名称等を通知すべき危険物及び有害物
キットコンポーネントの情報を参照してください
カルタヘナ法
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Jan Code
キットコンポーネントの情報を参照してください
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試験成績書（COA）
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
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How can I determine the shelf life / expiration / retest date of this product?
1 回答-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf役に立ちましたか？
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Can U or V bottom plates be used for taking the reading?
1 回答-
This kit requires the use of 96-well, clear, flat-bottom plates. Please see the link below to review the product Technical Bulletin for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/312/814/cs0003bul-ms.pdf役に立ちましたか？
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1. Is this kit suitable for estimation of red blood cell acetylcholinesterase activity? 2. Can Triton be used for haemolysate preparation?
1 回答-
This product is suitable for assaying acetylcholinesterase activity in cell lysates such as red blood cell hemolysates. Triton X-100 has not been tested for use in this kit. While there are published protocols that do suggest up to 1% Triton X-100, the impact if this detergent on results has not been evaluated. For cell lysates, it is recommended to prepare samples by brief sonication or by homogenization in 0.1 M phosphate buffer (pH 8.0), followed by centrifugation at 14,000 rpm for 5 minutes. The clear supernatant is used for the assay. The Assay Buffer included with the kit should be used for sample dilution.
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Is Acetylcholinesterase compatible with the Roche platform, specifically the COBAS 6000?
1 回答-
The product in question, an acetylcholinesterase enzyme or kit, is not compatible with the ROCHE Cobas 6000, as it is a clinical analyzer for clinical chemistry or immunochemistry. All acetylcholinesterase products available are intended for research purposes only and not for human or clinical use.
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Is this kit only for acetylcholinesterase or also tests for butylcholinesterase? Is this testing total cholinesterase activity or just AChE activity?
1 回答-
Product CS0003 only measures Acetylcholinesterase not butylcholinesterase and measures AChE activity.
役に立ちましたか？
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What is the difference between the two Acetylcholinesterase Activity Assay Kits MAK119 and CS0003?
1 回答-
Product CS0003 and MAK119 are produced by different manufacturers. In addition, CS0003 is sufficient for 200 tests while product MAK119 is sufficient for 100 tests. The principles and detection range are the same for both products.
The following is true for product CS0003:
- Direct procedure for measuring AChE levels in a variety of samples such as serum and plasma.
- Based on the Ellman method in which thiocholine, produced by AChE, reacts with 5,5'-dithiobis (2-nitrobenzoic acid) to form a colorimetric (412 nm) product, proportional to the AChE activity present.
- The AChE activity is measured without the use of a calibration curve. The AChE Positive Control in the kit can be used to screen for AChE inhibitors.
- This kit has a linear range of 10-600 units/liter of AChE activity.
The following is true for product MAK119:
- Direct assays of acetylcholinesterase activity in blood, serum, plasma, and other biological samples.
- Based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5’-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412 nm, is proportionate to the enzyme activity in the sample.
- Detection range 10 to 600 U/L AChE activity in 96-well plate assay.役に立ちましたか？
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Is product CS0003, Acetylcholinesterase Activity Assay Kit, suitable for use with whole blood? What is the recommended sample preparation protocol, e.g. the proper amount to dilute whole blood for assay?
1 回答-
Yes, kit CS0003 is suitable to test ACHE activity in whole blood. It is recommended to begin with (at least) 1:100 working dilution and progress from there.
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