This cell line is prepared from cultures grown in RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week. Bringing cells out of freeze in a new medium is not recommended. A sudden change of medium may result in poor expansion or viability. The end user would have to determine suitability of another media formulation. In such situations, it is best to transition cells gradually to a new culture medium by adding increasing proportions of the new medium to the old with each split or subculture. See the link below to the ECACC product datasheet for additional information:
https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=93112520&collection=ecacc_gc#
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製品名
A2780ADR Cell Line human, 93112520
由来生物
human ovary
詳細
Human Caucasian ovarian carcinoma
成長モード
Adherent
核型
Not specified
形態
Epithelial
製品
Not specified
受容体
Not specified
テクニック
cell culture | mammalian: suitable
関連疾患
cancer
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Human Caucasian ovarian carcinoma
細胞株の説明
The adriamycin-resistant cell line A2780ADR has been developed by exposure of the parent A2780 cell line (Sigma catalogue no. 93112519) to adriamycin. A2780ADR is cross-resistant to melphalan and vinblastine. To retain resistance adriamycin has to be added to the media. The cells grow as a monolayer and in suspension in spinner cultures and are tumourigenic in immune deficient mice. Together with the cisplatin-resistant variant A2780cis these lines only differ in their exposure to a single drug and should facilitate the search for molecular changes responsible for the expression of pleiotropic drug resistance in human ovarian cancer.
アプリケーション
Study of drug transport, toxicity testing, drug resistance cancer studies
DNAプロファイル
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 11,14
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 15,16
CSF1PO: 10,11
D13S317: 13
D16S539: 11,14
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 15,16
培地
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week.
継代と培養方法
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Recommendation: culture cells without drug after resuscitation until growth has been fully established.
その他情報
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免責事項
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
最新バージョンのいずれかを選択してください：
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Hi, I've just purchased the A2780ADR Cell Line human and had a few questions. Would there be any issues with using DMEM media instead of RPMI 1640 media? How is resistance measured in the cells likes and how do I insure that the resistance is retained?
1 回答-
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