2fTGH cell line human

12021508, human fibrosarcoma, Epithelial

• UNSPSCコード: 41106514

特性

• 製品名: 2fTGH cell line human, 12021508
• 由来生物: human fibrosarcoma
• 成長モード: adherent
• 形態: Epithelial
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の説明

The human sarcoma cell line HT 1080 (ECACC Catalogue number 85111505) was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by alpha interferon to create the 2fTGH cell line, enabling the selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the parent 2fTGH cell line enabled the isolation of a number of IFNg signalling mutants available from ECACC: U2A (12021502), U3A (12021503), U4A (12021504), U4C (12021505), U5A (12021506), U6A (12021507). Each contains a different mutation in the IFN signalling pathway. The 2fTGH panel provides a tool for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway.

DNAプロファイル

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

培地

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4 x 10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免責事項

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.