This product contains EDTA as the major active component, which acts as a chelating agent for divalent cations to loosen cell–cell contacts but does not break down the extracellular matrix as trypsin does. Empirical testing is needed to determine if this approach is more effective. It is highly recommended to review the literature to determine if an EDTA-only dissociation method has been successfully used with the particular primary microglia preparation protocol.
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品質水準
無菌性
sterile-filtered
製品種目
BioReagent
フォーム
solution
濃度
1 ×
テクニック
cell culture | mammalian: suitable
pH
6.5-7.5
輸送温度
wet ice
保管温度
room temp
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関連するカテゴリー
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
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Hi, I am working with primary microglia and it very tricky to dissociate. I usually dissociate them with 0.5% Trypsin for 7 min at 37˚C and still hard to collect them. May I know whether this reagent would be a better alternative? Thank you!
1 回答-
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What is the concentration of EDTA in Cat#C5789? Thanks
1 回答-
The concentration of EDTA in this formulation is proprietary. Additional components include Sodium Citrate, Nitrilotriacetic Acid, and Glycerol in Hank's Balanced Salt solution. Should the concentration of EDTA be required, product E8008, EDTA 0.2% in DPBS, may be of interest. Both items are tested for cell dissociation. Please see the link below to review this product option:
https://www.sigmaaldrich.com/US/en/product/sigma/e8008役に立ちましたか？
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